ArticlePloS one2015
The KLP-7 Residue S546 Is a Putative Aurora Kinase Site Required for Microtubule Regulation at the Centrosome in C. elegans.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 45 citations in OpenAlex.
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- Mechanical coordination between anaphase A and B drives asymmetric chromosome segregation.bioRxiv : the preprint server for biology · 2025Article
- Spatiotemporal single-cell architecture of gene expression in the Caenorhabditis elegans germ cells.Cell discovery · 2025Article
- Asymmetry in centrosome maturation revealed through AIR-1 dynamics in the early Caenorhabditis elegans embryo.Scientific reports · 2025Article
- The doublecortin-family kinase ZYG-8DCLK1 regulates microtubule dynamics and motor-driven forces to promote the stability of C. elegans acentrosomal spindles.PLoS genetics · 2024Article
- The Caenorhabditis elegans Shugoshin regulates TAC-1 in cilia.Scientific reports · 2023Article
- BUB-1 and CENP-C recruit PLK-1 to control chromosome alignment and segregation during meiosis I ineLife · 2023Article
- ZYG-9ch-TOG promotes the stability of acentrosomal poles via regulation of spindle microtubules in C. elegans oocyte meiosis.PLoS genetics · 2022Article
- Article
- A degron-based strategy reveals new insights into Aurora B function in C. elegans.PLoS genetics · 2021Article
- Evidence for anaphase pulling forces during C. elegans meiosis.The Journal of cell biology · 2020Article
- Excess crossovers impede faithful meiotic chromosome segregation in C. elegans.PLoS genetics · 2020Article
- Polar relaxation by dynein-mediated removal of cortical myosin II.The Journal of cell biology · 2020Article
- Male meiotic spindle features that efficiently segregate paired and lagging chromosomes.eLife · 2020Article
- Cell polarity-dependent centrosome separation in theThe Journal of cell biology · 2019Article
- Spindle assembly and chromosome dynamics during oocyte meiosis.Current opinion in cell biology · 2019Review
- Sumoylation regulates protein dynamics during meiotic chromosome segregation inJournal of cell science · 2019Article
- Kinetochore Recruitment of the Spindle and Kinetochore-Associated (Ska) Complex Is Regulated by Centrosomal PP2A inGenetics · 2019Article
- Dynamic SUMO remodeling drives a series of critical events during the meiotic divisions in Caenorhabditis elegans.PLoS genetics · 2018Article
- Fluorescence techniques in developmental biology.Journal of biosciences · 2018Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Regulation of microtubule dynamics is essential for many cellular processes, including proper assembly and function of the mitotic spindle. The kinesin-13 microtubule-depolymerizing enzymes provide one mechanism to regulate microtubule behaviour temporally and spatially. Vertebrate MCAK locates to chromatin, kinetochores, spindle poles, microtubule tips, and the cytoplasm, implying that the regulation of kinesin-13 activity and subcellular targeting is complex. Phosphorylation of kinesin-13 by Aurora kinase inhibits microtubule depolymerization activity and some Aurora phosphorylation sites on kinesin-13 are required for subcellular localization. Herein, we determine that a C. elegans deletion mutant klp-7(tm2143) causes meiotic and mitotic defects that are consistent with an increase in the amount of microtubules in the cytoplasmic and spindle regions of meiotic embryos, and an increase in microtubules emanating from centrosomes. We show that KLP-7 is phosphorylated by Aurora A and Aurora B kinases in vitro, and that the phosphorylation by Aurora A is stimulated by TPXL-1. Using a structure-function approach, we establish that one putative Aurora kinase site, S546, within the C-terminal part of the core domain is required for the function, but not subcellular localization, of KLP-7 in vivo. Furthermore, FRAP analysis reveals microtubule-dependent differences in the turnover of KLP-7(S546A) and KLP-7(S546E) mutant proteins at the centrosome, suggesting a possible mechanism for the regulation of KLP-7 by Aurora kinase.
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