ArticleStem cells international2015
Development of a System and Method for Automated Isolation of Stromal Vascular Fraction from Adipose Tissue Lipoaspirate.
Article in Stem cells international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- Influence of Donor Age, Donor Body Mass Index, and Harvesting Site on Cell Preparations from Human Adipose Tissue.International journal of molecular sciences · 2026Article
- Stem-Cell-Assisted Lipotransfer and Platelet-Rich Plasma in Breast Reconstruction: A Literature Review.Aesthetic plastic surgery · 2025Review
- Optimal Sca-1-based procedure for purifying mouse adipose-derived mesenchymal stem cells with enhanced proliferative and differentiation potential.Frontiers in cell and developmental biology · 2025Article
- Clinical applications of adipose-derived stromal vascular fraction in veterinary practice.The veterinary quarterly · 2022Article
- Autologous Adipose-Derived Tissue Stromal Vascular Fraction (AD-tSVF) for Knee Osteoarthritis.International journal of molecular sciences · 2022Review
- Modified nanofat grafting: Stromal vascular fraction simple and efficient mechanical isolation technique and perspectives in clinical recellularization applications.Frontiers in bioengineering and biotechnology · 2022Article
- Translational products of adipose tissue-derived mesenchymal stem cells: Bench to bedside applications.World journal of stem cells · 2021Review
- Inter-center comparison of good manufacturing practices-compliant stromal vascular fraction and proposal for release acceptance criteria: a review of 364 productions.Stem cell research & therapy · 2021Review
- Article
- Cellular Optimization of Nanofat: Comparison of Two Nanofat Processing Devices in Terms of Cell Count and Viability.Aesthetic surgery journal. Open forum · 2019Article
- Stromal Vascular Fraction and its Role in the Management of Alopecia: A Review.The Journal of clinical and aesthetic dermatology · 2019Review
- Isolation of adipose tissue derived regenerative cells from human subcutaneous tissue with or without the use of an enzymatic reagent.PloS one · 2019Article
- Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free media.Stem cell research & therapy · 2018Article
- Article
- Adipose tissue-derived stromal vascular fraction in regenerative medicine: a brief review on biology and translation.Stem cell research & therapy · 2017Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Autologous fat grafting for soft tissue reconstruction is challenged by unpredictable long-term graft survival. Fat derived stromal vascular fraction (SVF) is gaining popularity in tissue reconstruction as SVF-enriched fat grafts demonstrate improved engraftment. SVF also has potential in regenerative medicine for remodeling of ischemic tissues by promoting angiogenesis. Since SVF cells do not require culture expansion, attempts are being made to develop automated devices to isolate SVF at the point of care. We report development of a closed, automated system to process up to 500 mL lipoaspirate using cell size-dependent filtration technology. The yield of SVF obtained by automated tissue digestion and filtration (1.17 ± 0.5 × 10(5) cells/gram) was equivalent to that obtained by manual isolation (1.15 ± 0.3 × 10(5); p = 0.8), and the viability of the cells isolated by both methods was greater than 90%. Cell composition included CD34+CD31- adipose stromal cells, CD34+CD31+ endothelial progenitor cells, and CD34-CD31+ endothelial cells, and their relative percentages were equivalent to SVF isolated by the manual method. CFU-F capacity and expression of angiogenic factors were also comparable with the manual method, establishing proof-of-concept for fully automated SVF isolation, suitable for use in reconstructive surgeries and regenerative medicine applications.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.