ArticleFrontiers in microbiology2015
The primary pathway for lactate oxidation in Desulfovibrio vulgaris.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 60 citations in OpenAlex.
- Physiological characterization of anaerobic cometabolic transformation of sulfamethoxazole by Nitratidesulfovibrio vulgaris Hildenborough.Applied microbiology and biotechnology · 2026Article
- Comparative proteomics reveals hydrogenase-centered steel-dependent growth and corrosion inFrontiers in microbiology · 2026Article
- Postbiotic studies of mixed cultures ofVeterinary world · 2024Article
- Origin of biogeographically distinct ecotypes during laboratory evolution.Nature communications · 2024Article
- Combining metabolic flux analysis with proteomics to shed light on the metabolic flexibility: the case ofFrontiers in microbiology · 2024Article
- Transcriptome-wide marker gene expression analysis of stress-responsive sulfate-reducing bacteria.Scientific reports · 2023Article
- Pyridoxal 5'-phosphate synthesis and salvage in Bacteria and Archaea: predicting pathway variant distributions and holes.Microbial genomics · 2023Article
- Large-scale genetic characterization of the model sulfate-reducing bacterium,Frontiers in microbiology · 2023Article
- Inhibition of Sulfate Reduction and Cell Division by Desulfovibrio desulfuricans Coated in Palladium Metal.Applied and environmental microbiology · 2022Article
- Article
- Influence of yeast on rumen fermentation, growth performance and quality of products in ruminants: A review.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2021Review
- Key Enzymes for Anaerobic Lactate Metabolism in Geobacter sulfurreducens.Applied and environmental microbiology · 2021Article
- Organohalide-respiring Desulfoluna species isolated from marine environments.The ISME journal · 2020Article
- Comparative Genomics Reveals Metabolic Specificity ofMicroorganisms · 2019Article
- Effects of live yeast on differential genetic and functional attributes of rumen microbiota in beef cattle.Journal of animal science and biotechnology · 2019Article
- Proteomic and Isotopic Response ofFrontiers in microbiology · 2019Article
- LurR is a regulator of the central lactate oxidation pathway in sulfate-reducing Desulfovibrio species.PloS one · 2019Article
- Methane-yielding microbial communities processing lactate-rich substrates: a piece of the anaerobic digestion puzzle.Biotechnology for biofuels · 2018Article
- Acylation of the Type 3 Secretion System Translocon Using a Dedicated Acyl Carrier Protein.PLoS genetics · 2017Article
- Biochemical Function, Molecular Structure and Evolution of an Atypical Thioredoxin Reductase fromFrontiers in microbiology · 2017Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ability to respire sulfate linked to lactate oxidation is a key metabolic signature of the Desulfovibrio genus. Lactate oxidation by these incomplete oxidizers generates reductants through lactate dehydrogenase (LDH) and pyruvate-ferredoxin oxidoreductase (PFOR), with the latter catalyzing pyruvate conversion into acetyl-CoA. Acetyl-CoA is the source of substrate-level phosphorylation through the production of ATP. Here, we show that these crucial steps are performed by enzymes encoded by a nonacistronic transcriptional unit named now as operon luo (for lactate utilization operon). Using a combination of genetic and biochemical techniques, we assigned a physiological role to the operon genes DVU3027-28 and DVU3032-33. The growth of mutant Δ26-28 was highly disrupted on D-lactate, whereas the growth of mutant Δ32-33 was slower on L-lactate, which could be related to a decrease in the activity of D-lactate or L-lactate oxidase in the corresponding mutants. The DVU3027-28 and DVU3032-33 genes thus encode functional D-LDH and L-LDH enzymes, respectively. Scanning of the genome for lactate utilization revealed several lactate permease and dehydrogenase homologs. However, transcriptional compensation was not observed in any of the mutants except for lactate permease. Although there is a high degree of redundancy for lactate oxidase, it is not functionally efficient in LDH mutants. This result could be related to the identification of several operon enzymes, including LDHs, in the PFOR activity bands, suggesting the occurrence of a lactate-oxidizing supermolecular structure that can optimize the performance of lactate utilization in Desulfovibrio species.
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