ArticleJournal of genetics and genomics = Yi chuan xue bao2015
Fast-suppressor screening for new components in protein trafficking, organelle biogenesis and silencing pathway in Arabidopsis thaliana using DEX-inducible FREE1-RNAi plants.
Article in Journal of genetics and genomics = Yi chuan xue bao, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- ESCRTs in plant abiotic stresses.Essays in biochemistry · 2025Review
- Genetic Screening of Factors in the Plant Protein Secretion.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The plant-unique protein DRIF1 coordinates with sorting nexin 1 to regulate membrane protein homeostasis.The Plant cell · 2023Article
- Alkaline stress reduces root waving by regulating PIN7 vacuolar transport.Frontiers in plant science · 2022Article
- RST1 Is a FREE1 Suppressor That Negatively Regulates Vacuolar Trafficking in Arabidopsis.The Plant cell · 2019Article
- The plant ESCRT component FREE1 shuttles to the nucleus to attenuate abscisic acid signalling.Nature plants · 2019Article
- Genetic Suppressor Screen Using an Inducible FREE1-RNAi Line to Detect ESCRT Genetic Interactors in Arabidopsis thaliana.Methods in molecular biology (Clifton, N.J.) · 2019Article
- A plant Bro1 domain protein BRAF regulates multivesicular body biogenesis and membrane protein homeostasis.Nature communications · 2018Article
- Dynamics of Autophagosome Formation.Plant physiology · 2018Review
- MONENSIN SENSITIVITY1 (MON1)/CALCIUM CAFFEINE ZINC SENSITIVITY1 (CCZ1)-Mediated Rab7 Activation Regulates Tapetal Programmed Cell Death and Pollen Development.Plant physiology · 2017Article
- A Distinct Pathway for Polar Exocytosis in Plant Cell Wall Formation.Plant physiology · 2016Article
- Endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) in plants.Protoplasma · 2016Review
- Exocyst-Positive Organelles and Autophagosomes Are Distinct Organelles in Plants.Plant physiology · 2015Article
Corrections and comments
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Authors and funding
23 authors at 7 institutions in 2 countries.
Funding
Abstract
Membrane trafficking is essential for plant growth and responses to external signals. The plant unique FYVE domain-containing protein FREE1 is a component of the ESCRT complex (endosomal sorting complex required for transport). FREE1 plays multiple roles in regulating protein trafficking and organelle biogenesis including the formation of intraluminal vesicles of multivesicular body (MVB), vacuolar protein transport and vacuole biogenesis, and autophagic degradation. FREE1 knockout plants show defective MVB formation, abnormal vacuolar transport, fragmented vacuoles, accumulated autophagosomes, and seedling lethality. To further uncover the underlying mechanisms of FREE1 function in plants, we performed a forward genetic screen for mutants that suppressed the seedling lethal phenotype of FREE1-RNAi transgenic plants. The obtained mutants are termed as suppressors of free1 (sof). To date, 229 putative sof mutants have been identified. Barely detecting of FREE1 protein with M3 plants further identified 84 FREE1-related suppressors. Also 145 mutants showing no reduction of FREE1 protein were termed as RNAi-related mutants. Through next-generation sequencing (NGS) of bulked DNA from F2 mapping population of two RNAi-related sof mutants, FREE1-RNAi T-DNA inserted on chromosome 1 was identified and the causal mutation of putative sof mutant is being identified similarly. These FREE1- and RNAi-related sof mutants will be useful tools and resources for illustrating the underlying mechanisms of FREE1 function in intracellular trafficking and organelle biogenesis, as well as for uncovering the new components involved in the regulation of silencing pathways in plants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.