ArticleJournal of genetics and genomics = Yi chuan xue bao2015
A genome-wide CRISPR library for high-throughput genetic screening in Drosophila cells.
Article in Journal of genetics and genomics = Yi chuan xue bao, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed.
- Dual-line Genome-scale CRISPR Screening Enables Robust Target Gene Discovery.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Identification ofFrontiers in immunology · 2026Review
- Single amino-acid mutation in a Drosoph ila melanogaster ribosomal protein: An insight in uL11 transcriptional activity.PloS one · 2022Article
- A metabolic CRISPR-Cas9 screen in Chinese hamster ovary cells identifies glutamine-sensitive genes.Metabolic engineering · 2021Article
- Expansion of the CRISPR/Cas Genome-Sculpting Toolbox: Innovations, Applications and Challenges.Molecular diagnosis & therapy · 2021Review
- An inducible CRISPR interference library for genetic interrogation of Saccharomyces cerevisiae biology.Communications biology · 2020Article
- Anchor Away - A Fast, Reliable and Reversible Technique To Inhibit Proteins inG3 (Bethesda, Md.) · 2020Article
- Genome-wide CRISPR screening reveals genes essential for cell viability and resistance to abiotic and biotic stresses inGenome research · 2020Article
- Article
- Generating and working with Drosophila cell cultures: Current challenges and opportunities.Wiley interdisciplinary reviews. Developmental biology · 2019Review
- The length of guide RNA and target DNA heteroduplex effects on CRISPR/Cas9 mediated genome editing efficiency in porcine cells.Journal of veterinary science · 2019Article
- HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries.Journal of visualized experiments : JoVE · 2019Article
- Responsive Cells for rhEGF bioassay Obtained through Screening of a CRISPR/Cas9 Library.Scientific reports · 2019Article
- Double Selection Enhances the Efficiency of Target-AID and Cas9-Based Genome Editing in Yeast.G3 (Bethesda, Md.) · 2018Article
- Pooled genome-wide CRISPR screening for basal and context-specific fitness gene essentiality ineLife · 2018Article
- Review
- ENCoRE: an efficient software for CRISPR screens identifies new players in extrinsic apoptosis.BMC genomics · 2017Article
- Article
- A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer.Journal of visualized experiments : JoVE · 2017Article
- CRISPR/Cas9: Transcending the Reality of Genome Editing.Molecular therapy. Nucleic acids · 2017Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The simplicity of the CRISPR/Cas9 system of genome engineering has opened up the possibility of performing genome-wide targeted mutagenesis in cell lines, enabling screening for cellular phenotypes resulting from genetic aberrations. Drosophila cells have proven to be highly effective in identifying genes involved in cellular processes through similar screens using partial knockdown by RNAi. This is in part due to the lower degree of redundancy between genes in this organism, whilst still maintaining highly conserved gene networks and orthologs of many human disease-causing genes. The ability of CRISPR to generate genetic loss of function mutations not only increases the magnitude of any effect over currently employed RNAi techniques, but allows analysis over longer periods of time which can be critical for certain phenotypes. In this study, we have designed and built a genome-wide CRISPR library covering 13,501 genes, among which 8989 genes are targeted by three or more independent single guide RNAs (sgRNAs). Moreover, we describe strategies to monitor the population of guide RNAs by high throughput sequencing (HTS). We hope that this library will provide an invaluable resource for the community to screen loss of function mutations for cellular phenotypes, and as a source of guide RNA designs for future studies.
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Registered trials
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