Evidence map›Paper›PMID 26164699›Full record

ArticleMethods (San Diego, Calif.)2015

A genome-wide RNAi screening method to discover novel genes involved in virus infection.

Debasis Panda, Sara Cherry

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods (San Diego, Calif.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.0field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

Debasis PandaLaboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, United States. Electronic address: debasis.panda@nih.gov.
Sara CherryDepartment of Microbiology, University of Pennsylvania, Philadelphia, PA 19104, United States. Electronic address: cherrys@mail.med.upenn.edu.
National Institute of Allergy and Infectious Diseases · USUniversity of Pennsylvania · US

Funding

Vaccine strategy for broad spectrum protection agains non-typhoidal salmonellU54AI057168 · NIAID · UNIVERSITY OF MARYLAND BALTIMORE · PI LEVINE, MYRON MAX · 2003 to 2013
$91.5M
The role of autophagy in innate anti-viral immunity in DrosophilaR01AI074951 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI CHERRY, SARA · 2007 to 2017
$4.2M
Dissecting the alphavirus entry receptor NRAMPR01AI095500 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI CHERRY, SARA · 2012 to 2015
$1.9M
Defining the determinants on the alphavirus receptor NRAMP required for virus binR21AI103441 · NIAID · UNIVERSITY OF PENNSYLVANIA · PI CHERRY, SARA · 2013 to 2014
$440k
Intramural NIH HHS Z99 AI999999NIAID NIH HHS R01 AI074951NIAID NIH HHS R01AI074951NIAID NIH HHS R01 AI095500NIAID NIH HHS R01AI095500NIAID NIH HHS R21 AI103441NIAID NIH HHS R21AI103441NIAID NIH HHS U54 AI057168NIAID NIH HHS U54AI057168
6 · The paper itself

Abstract

Systematic and comprehensive analysis of host cell proteins involved in virus infection has been difficult in large part due to the lack of robust unbiased methods for their identification. Recent technological breakthroughs allowing development of cell-based genetic screens have greatly facilitated our understanding of virus-host interactions. These include instrumentation for processing in microtiter plates (e.g., 384 well), coupled with sensitive readers and off-the-shelf analysis and informatics pipelines. Because viruses are a significant threat to human health, a better understanding of the cellular factors that impact infection would pave the way for the development of new therapeutics. Here we describe the development and implementation of a genome-wide siRNA screen against a virus using human cells.

Indexed as

Genes, ViralRNA InterferenceGenome, ViralHumansSequence Analysis, DNAVirusesArrayed screenHigh-content screeningImage-based screeningsiRNA screening protocolVirus–host interactions

Identifiers

PMID26164699
PMCPMC4684736
OpenAlexW775964593

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.