ArticleAnesthesiology2015
Silencing the α2 subunit of γ-aminobutyric acid type A receptors in rat dorsal root ganglia reveals its major role in antinociception posttraumatic nerve injury.
Article in Anesthesiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 26 citations in OpenAlex.
- Glial cells in neuropathic pain.Physiological reviews · 2026Review
- Peripheral gating of mechanosensation by glial diazepam binding inhibitor.The Journal of clinical investigation · 2024Article
- The Role of Neuroactive Steroids in Analgesia and Anesthesia: An Interesting Comeback?Biomolecules · 2023Review
- Dorsal root ganglia control nociceptive input to the central nervous system.PLoS biology · 2023Article
- Positive interaction between GPER and β-alanine in the dorsal root ganglion uncovers potential mechanisms: mediating continuous neuronal sensitization and neuroinflammation responses in neuropathic pain.Journal of neuroinflammation · 2022Article
- Thoracic Jia-Ji electro-acupuncture mitigates low skeletal muscle atrophy and improves motor function recovery following thoracic spinal cord injury in rats.American journal of translational research · 2022Article
- Presynaptic Inhibition of Pain and Touch in the Spinal Cord: From Receptors to Circuits.International journal of molecular sciences · 2021Review
- Neuropathic Injury-Induced Plasticity of GABAergic System in Peripheral Sensory Ganglia.Frontiers in pharmacology · 2021Article
- Role of GABAAR in the Transition From Acute to Chronic Pain and the Analgesic Effect of Electroacupuncture on Hyperalgesic Priming Model Rats.Frontiers in neuroscience · 2021Article
- Pain condition and sex differences in the descending noradrenergic system following lateral hypothalamic stimulation.IBRO reports · 2020Article
- Chloride - The Underrated Ion in Nociceptors.Frontiers in neuroscience · 2020Review
- Mechanism of dorsal root ganglion stimulation for pain relief in painful diabetic polyneuropathy is not dependent on GABA release in the dorsal horn of the spinal cord.CNS neuroscience & therapeutics · 2020Article
- Targeting Peripheral Somatosensory Neurons to Improve Tactile-Related Phenotypes in ASD Models.Cell · 2019Article
- GABA-B1 Receptor-Null Schwann Cells Exhibit Compromised In Vitro Myelination.Molecular neurobiology · 2019Article
- Neurosteroids in Pain Management: A New Perspective on an Old Player.Frontiers in pharmacology · 2018Review
- Local GABAergic signaling within sensory ganglia controls peripheral nociceptive transmission.The Journal of clinical investigation · 2017Article
- Tail Nerve Electrical Stimulation and Electro-Acupuncture Can Protect Spinal Motor Neurons and Alleviate Muscle Atrophy after Spinal Cord Transection in Rats.Neural plasticity · 2017Article
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundNeuropathic pain (NPP) is likely the result of repetitive high-frequency bursts of peripheral afferent activity leading to long-lasting changes in synaptic plasticity in the spinal dorsal horn. Drugs that promote γ-aminobutyric acid (GABA) activity in the dorsal horn provide partial relief of neuropathic symptoms. The authors examined how in vivo silencing of the GABA receptor type A (GABAA) α2 gene in dorsal root ganglia (DRG) controls NPP.
methodsAfter crush injury to the right sciatic nerve of female rats, the α2 GABAA antisense and mismatch oligodeoxynucleotides or NO-711 (a GABA uptake inhibitor) were applied to the L5 DRG. In vivo behavioral assessment of nociception was conducted before the injury and ensuing 10 days (n = 4 to 10). In vitro quantification of α2 GABAA protein and electrophysiological studies of GABAA currents were performed on acutely dissociated L5 DRG neurons at relevant time points (n = 6 to 14).
resultsNPP postcrush injury of a sciatic nerve in adult female rats coincides with significant down-regulation of the α2 subunit expression in the ipsilateral DRG (approximately 30%). Selective down-regulation of α2 expression in DRGs significantly worsens mechanical (2.55 ± 0.75 to 5.16 ± 1.16) and thermal (7.97 ± 0.96 to 5.51 ± 0.75) hypersensitivity in crush-injured animals and causes development of significant mechanical (2.33 ± 0.40 to 5.00 ± 0.33) and thermal (10.80 ± 0.29 to 7.34 ± 0.81) hypersensitivity in sham animals (data shown as mean ± SD). Conversely, up-regulation of endogenous GABA via blockade of its uptake in DRG alleviates NPP.
conclusionThe GABAA receptor in the DRG plays an important role in pathophysiology of NPP caused by sciatic nerve injury and represents promising target for novel pain therapies.
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