ArticleBiosensors & bioelectronics2015
A portable, shock-proof, surface-heated droplet PCR system for Escherichia coli detection.
Article in Biosensors & bioelectronics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
11 citing papers in PubMed, 45 citations in OpenAlex.
- Recent Advances in Digital Biosensing Technology.Biosensors · 2022Review
- Machine learning classification of bacterial species using mix-and-match reagents on paper microfluidic chips and smartphone-based capillary flow analysis.Analytical and bioanalytical chemistry · 2022Article
- Microheater: material, design, fabrication, temperature control, and applications-a role in COVID-19.Biomedical microdevices · 2021Review
- A plasmonic gold nanofilm-based microfluidic chip for rapid and inexpensive droplet-based photonic PCR.Scientific reports · 2021Article
- All-fiber all-optical quantitative polymerase chain reaction (qPCR).Sensors and actuators. B, Chemical · 2020Article
- Smartphone operable centrifugal system (SOCS) for on-site DNA extraction from foodborne bacterial pathogen.Biomicrofluidics · 2019Article
- Thermal sensing in fluid at the micro-nano-scales.Biomicrofluidics · 2018Review
- Sensors Based on Bio and Biomimetic Receptors in Medical Diagnostic, Environment, and Food Analysis.Biosensors · 2018Review
- Electrical Tweezer for Droplet Transportation, Extraction, Merging and DNA Analysis.Micromachines · 2017Article
- In situ, dual-mode monitoring of organ-on-a-chip with smartphone-based fluorescence microscope.Biosensors & bioelectronics · 2016Article
- Novel developments in mobile sensing based on the integration of microfluidic devices and smartphones.Lab on a chip · 2016Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
A novel polymerase chain reaction (PCR) device was developed that uses wire-guided droplet manipulation (WDM) to guide a droplet over three different heating chambers. After PCR amplification, end-point detection is achieved using a smartphone-based fluorescence microscope. The device was tested for identification of the 16S rRNA gene V3 hypervariable region from Escherichia coli genomic DNA. The lower limit of detection was 10(3) genome copies per sample. The device is portable with smartphone-based end-point detection and provides the assay results quickly (15 min for a 30-cycle amplification) and accurately. The system is also shock and vibration resistant, due to the multiple points of contact between the droplet and the thermocouple and the Teflon film on the heater surfaces. The thermocouple also provides real-time droplet temperature feedback to ensure it reaches the set temperature before moving to the next chamber/step in PCR. The device is equipped to use either silicone oil or coconut oil. Coconut oil provides additional portability and ease of transportation by eliminating spilling because its high melting temperature means it is solid at room temperature.
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Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.