ArticleEukaryotic cell2015
Analysis of the Aspergillus fumigatus Biofilm Extracellular Matrix by Solid-State Nuclear Magnetic Resonance Spectroscopy.
Article in Eukaryotic cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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Who cites it
43 citing papers in PubMed, 85 citations in OpenAlex.
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- Biofilm Formation inMicroorganisms · 2026Article
- Proteomic and Phosphoproteomic Landscapes of Azole Resistance in Aspergillus fumigatus Biofilm Exposed to Voriconazole.Mycopathologia · 2025Article
- Ultrasensitive Characterization of Native Bacterial Biofilms via Dynamic Nuclear Polarization-Enhanced Solid-State NMR.Angewandte Chemie (International ed. in English) · 2025Article
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- The hidden role of heterotrophic bacteria in early carbonate diagenesis.Scientific reports · 2025Article
- Heterologous Expression of Plantaricin 423 and Mundticin ST4SA in Saccharomyces cerevisiae.Probiotics and antimicrobial proteins · 2024Article
- New Vision of Cell Walls inJournal of fungi (Basel, Switzerland) · 2024Review
- Characterization of the broad-spectrum antibacterial activity of bacteriocin-like inhibitory substance-producing probiotics isolated from fermented foods.BMC microbiology · 2024Article
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- Interactions between Bacteria andJournal of fungi (Basel, Switzerland) · 2023Review
- Biotechnological applications of biofilms formed by osmotolerant and halotolerant yeasts.Applied microbiology and biotechnology · 2023Review
- The Determination, Monitoring, Molecular Mechanisms and Formation of Biofilm in E. coli.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Review
- Solid-State NMR Investigations of Extracellular Matrixes and Cell Walls of Algae, Bacteria, Fungi, and Plants.Chemical reviews · 2022Review
- Solid-state NMR analysis of unlabeled fungal cell walls fromJournal of structural biology: X · 2022Article
- Organic composition of epilithic biofilms from agricultural and urban watershed in South Brazil.Environmental science and pollution research international · 2021Article
- Molecular Modifications of the Pseudomonas Quinolone Signal in the Intermicrobial Competition withJournal of fungi (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Aspergillus fumigatus is commonly responsible for lethal fungal infections among immunosuppressed individuals. A. fumigatus forms biofilm communities that are of increasing biomedical interest due to the association of biofilms with chronic infections and their increased resistance to antifungal agents and host immune factors. Understanding the composition of microbial biofilms and the extracellular matrix is important to understanding function and, ultimately, to developing strategies to inhibit biofilm formation. We implemented a solid-state nuclear magnetic resonance (NMR) approach to define compositional parameters of the A. fumigatus extracellular matrix (ECM) when biofilms are formed in RPMI 1640 nutrient medium. Whole biofilm and isolated matrix networks were also characterized by electron microscopy, and matrix proteins were identified through protein gel analysis. The (13)C NMR results defined and quantified the carbon contributions in the insoluble ECM, including carbonyls, aromatic carbons, polysaccharide carbons (anomeric and nonanomerics), aliphatics, etc. Additional (15)N and (31)P NMR spectra permitted more specific annotation of the carbon pools according to C-N and C-P couplings. Together these data show that the A. fumigatus ECM produced under these growth conditions contains approximately 40% protein, 43% polysaccharide, 3% aromatic-containing components, and up to 14% lipid. These fundamental chemical parameters are needed to consider the relationships between composition and function in the A. fumigatus ECM and will enable future comparisons with other organisms and with A. fumigatus grown under alternate conditions.
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Registered trials
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