ArticleGenetics2015
Auxin and Tryptophan Homeostasis Are Facilitated by the ISS1/VAS1 Aromatic Aminotransferase in Arabidopsis.
Article in Genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Herbivore cues and plant damage-associated compounds jointly alter seed germination and seedling herbivory.Oecologia · 2025Article
- QTL mapping and candidate gene identification of the full flowering date trait in olive trees (Olea Europaea L.).BMC plant biology · 2025Article
- Mapping multi-substrate specificity of Arabidopsis aminotransferases.Nature plants · 2025Article
- Multisubstrate specificity shaped the complex evolution of the aminotransferase family across the tree of life.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- An auxin research odyssey: 1989-2023.The Plant cell · 2024Review
- The cytosolic aminotransferase VAS1 coordinates aromatic amino acid biosynthesis and metabolism.Science advances · 2024Article
- Analytical methods for stable isotope labeling to elucidate rapid auxin kinetics in Arabidopsis thaliana.PloS one · 2024Article
- Comparative Coexpression Analysis of Indole Synthase and Tryptophan Synthase A Reveals the Independent Production of Auxin via the Cytosolic Free Indole.Plants (Basel, Switzerland) · 2023Article
- Atypical ageing defect in Pinot Blanc wines: a comparison between organic and conventional production management systems.Journal of the science of food and agriculture · 2023Article
- Evidence from Co-expression Analysis for the Involvement of Amidase and INS in the Tryptophan-Independent Pathway of IAA Synthesis in Arabidopsis.Applied biochemistry and biotechnology · 2022Article
- Protocol: analytical methods for visualizing the indolic precursor network leading to auxin biosynthesis.Plant methods · 2021Article
- Natural allelic variation in a modulator of auxin homeostasis improves grain yield and nitrogen use efficiency in rice.The Plant cell · 2021Article
- Loss of function of theProceedings of the National Academy of Sciences of the United States of America · 2020Article
- The Role of Auxin-Ethylene Crosstalk in Orchestrating Primary Root Elongation in Sugar Beet.Frontiers in plant science · 2017Article
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
Indole-3-acetic acid (IAA) plays a critical role in regulating numerous aspects of plant growth and development. While there is much genetic support for tryptophan-dependent (Trp-D) IAA synthesis pathways, there is little genetic evidence for tryptophan-independent (Trp-I) IAA synthesis pathways. Using Arabidopsis, we identified two mutant alleles of ISS1 ( I: ndole S: evere S: ensitive) that display indole-dependent IAA overproduction phenotypes including leaf epinasty and adventitious rooting. Stable isotope labeling showed that iss1, but not WT, uses primarily Trp-I IAA synthesis when grown on indole-supplemented medium. In contrast, both iss1 and WT use primarily Trp-D IAA synthesis when grown on unsupplemented medium. iss1 seedlings produce 8-fold higher levels of IAA when grown on indole and surprisingly have a 174-fold increase in Trp. These findings indicate that the iss1 mutant's increase in Trp-I IAA synthesis is due to a loss of Trp catabolism. ISS1 was identified as At1g80360, a predicted aromatic aminotransferase, and in vitro and in vivo analysis confirmed this activity. At1g80360 was previously shown to primarily carry out the conversion of indole-3-pyruvic acid to Trp as an IAA homeostatic mechanism in young seedlings. Our results suggest that in addition to this activity, in more mature plants ISS1 has a role in Trp catabolism and possibly in the metabolism of other aromatic amino acids. We postulate that this loss of Trp catabolism impacts the use of Trp-D and/or Trp-I IAA synthesis pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.