ArticleApplied and environmental microbiology2015
Rerouting Cellular Electron Flux To Increase the Rate of Biological Methane Production.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 21 citations in OpenAlex.
- Overexpression of 2-mercaptoethanesulfonate biosynthesis genesJournal of bacteriology · 2025Article
- Mer overexpression inApplied and environmental microbiology · 2025Article
- Model Organisms To Study Methanogenesis, a Uniquely Archaeal Metabolism.Journal of bacteriology · 2023Review
- Supplementation of Sulfide or Acetate and 2-Mercaptoethane Sulfonate Restores Growth of theMicroorganisms · 2023Article
- Reintegrating Biology Through the Nexus of Energy, Information, and Matter.Integrative and comparative biology · 2022Article
- Insights into the biotechnology potential ofFrontiers in microbiology · 2022Review
- Anaerobic Production of Isoprene by EngineeredApplied and environmental microbiology · 2021Article
- Review
- Methanogens: pushing the boundaries of biology.Emerging topics in life sciences · 2018Article
- Metabolic shift at the class level sheds light on adaptation of methanogens to oxidative environments.The ISME journal · 2018Article
- Physiological Evidence for Isopotential Tunneling in the Electron Transport Chain of Methane-Producing Archaea.Applied and environmental microbiology · 2017Article
- Genomic analysis of methanogenic archaea reveals a shift towards energy conservation.BMC genomics · 2017Article
- Article
- Genome-Scale Metabolic Modeling of Archaea Lends Insight into Diversity of Metabolic Function.Archaea (Vancouver, B.C.) · 2017Review
- Genome-wide gene expression and RNA half-life measurements allow predictions of regulation and metabolic behavior in Methanosarcina acetivorans.BMC genomics · 2016Article
- Methods for Detecting Microbial Methane Production and Consumption by Gas Chromatography.Bio-protocol · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Methanogens are anaerobic archaea that grow by producing methane, a gas that is both an efficient renewable fuel and a potent greenhouse gas. We observed that overexpression of the cytoplasmic heterodisulfide reductase enzyme HdrABC increased the rate of methane production from methanol by 30% without affecting the growth rate relative to the parent strain. Hdr enzymes are essential in all known methane-producing archaea. They function as the terminal oxidases in the methanogen electron transport system by reducing the coenzyme M (2-mercaptoethane sulfonate) and coenzyme B (7-mercaptoheptanoylthreonine sulfonate) heterodisulfide, CoM-S-S-CoB, to regenerate the thiol-coenzymes for reuse. In Methanosarcina acetivorans, HdrABC expression caused an increased rate of methanogenesis and a decrease in metabolic efficiency on methylotrophic substrates. When acetate was the sole carbon and energy source, neither deletion nor overexpression of HdrABC had an effect on growth or methane production rates. These results suggest that in cells grown on methylated substrates, the cell compensates for energy losses due to expression of HdrABC with an increased rate of substrate turnover and that HdrABC lacks the appropriate electron donor in acetate-grown cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.