Evidence map›Paper›PMID 26162429›Full record

ArticlePlant physiology2015

RNA Interference Knockdown of BRASSINOSTEROID INSENSITIVE1 in Maize Reveals Novel Functions for Brassinosteroid Signaling in Controlling Plant Architecture.

Gokhan Kir, Huaxun Ye, Hilde Nelissen, Anjanasree K Neelakandan, Andree S Kusnandar, Anding Luo, Dirk Inzé, Anne W Sylvester, Yanhai Yin, Philip W Becraft

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In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.

0numbers the graph read from it
0cells of the map it votes in
70citing papers in PubMed
14.5field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

70 citing papers in PubMed, 135 citations in OpenAlex.

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  17. Phenotypic characterization and genetic mapping of the semi-dwarf mutant sdw9 in maize.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024
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  18. Review
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  20. Review

10 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Gokhan KirGenetics, Development, and Cell Biology Department (G.K., H.Y., A.K.N., A.S.K., Y.Y., P.W.B.), Interdepartmental Genetics and Genomics Program (G.K., H.Y., A.S.K., Y.Y., P.W.B.), and Agronomy Department (P.W.B.), Iowa State University, Ames, Iowa 50011;Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, B-9052 Ghent, Belgium (H.N., D.I.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (H.N., D.I.); andDepartment of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071-2000 (A.L., A.W.S.).
Huaxun YeGenetics, Development, and Cell Biology Department (G.K., H.Y., A.K.N., A.S.K., Y.Y., P.W.B.), Interdepartmental Genetics and Genomics Program (G.K., H.Y., A.S.K., Y.Y., P.W.B.), and Agronomy Department (P.W.B.), Iowa State University, Ames, Iowa 50011;Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, B-9052 Ghent, Belgium (H.N., D.I.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (H.N., D.I.); andDepartment of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071-2000 (A.L., A.W.S.).ORCID http://orcid.org/0000-0001-9878-1447
Hilde NelissenGenetics, Development, and Cell Biology Department (G.K., H.Y., A.K.N., A.S.K., Y.Y., P.W.B.), Interdepartmental Genetics and Genomics Program (G.K., H.Y., A.S.K., Y.Y., P.W.B.), and Agronomy Department (P.W.B.), Iowa State University, Ames, Iowa 50011;Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, B-9052 Ghent, Belgium (H.N., D.I.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (H.N., D.I.); andDepartment of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071-2000 (A.L., A.W.S.).ORCID http://orcid.org/0000-0001-7494-1290
Anjanasree K NeelakandanGenetics, Development, and Cell Biology Department (G.K., H.Y., A.K.N., A.S.K., Y.Y., P.W.B.), Interdepartmental Genetics and Genomics Program (G.K., H.Y., A.S.K., Y.Y., P.W.B.), and Agronomy Department (P.W.B.), Iowa State University, Ames, Iowa 50011;Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, B-9052 Ghent, Belgium (H.N., D.I.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (H.N., D.I.); andDepartment of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071-2000 (A.L., A.W.S.).
Andree S KusnandarGenetics, Development, and Cell Biology Department (G.K., H.Y., A.K.N., A.S.K., Y.Y., P.W.B.), Interdepartmental Genetics and Genomics Program (G.K., H.Y., A.S.K., Y.Y., P.W.B.), and Agronomy Department (P.W.B.), Iowa State University, Ames, Iowa 50011;Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, B-9052 Ghent, Belgium (H.N., D.I.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (H.N., D.I.); andDepartment of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071-2000 (A.L., A.W.S.).
Anding LuoGenetics, Development, and Cell Biology Department (G.K., H.Y., A.K.N., A.S.K., Y.Y., P.W.B.), Interdepartmental Genetics and Genomics Program (G.K., H.Y., A.S.K., Y.Y., P.W.B.), and Agronomy Department (P.W.B.), Iowa State University, Ames, Iowa 50011;Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, B-9052 Ghent, Belgium (H.N., D.I.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (H.N., D.I.); andDepartment of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071-2000 (A.L., A.W.S.).ORCID http://orcid.org/0000-0002-2603-8854
Dirk InzéGenetics, Development, and Cell Biology Department (G.K., H.Y., A.K.N., A.S.K., Y.Y., P.W.B.), Interdepartmental Genetics and Genomics Program (G.K., H.Y., A.S.K., Y.Y., P.W.B.), and Agronomy Department (P.W.B.), Iowa State University, Ames, Iowa 50011;Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, B-9052 Ghent, Belgium (H.N., D.I.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (H.N., D.I.); andDepartment of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071-2000 (A.L., A.W.S.).
Anne W SylvesterGenetics, Development, and Cell Biology Department (G.K., H.Y., A.K.N., A.S.K., Y.Y., P.W.B.), Interdepartmental Genetics and Genomics Program (G.K., H.Y., A.S.K., Y.Y., P.W.B.), and Agronomy Department (P.W.B.), Iowa State University, Ames, Iowa 50011;Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, B-9052 Ghent, Belgium (H.N., D.I.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (H.N., D.I.); andDepartment of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071-2000 (A.L., A.W.S.).ORCID http://orcid.org/0000-0001-7282-4189
Yanhai YinGenetics, Development, and Cell Biology Department (G.K., H.Y., A.K.N., A.S.K., Y.Y., P.W.B.), Interdepartmental Genetics and Genomics Program (G.K., H.Y., A.S.K., Y.Y., P.W.B.), and Agronomy Department (P.W.B.), Iowa State University, Ames, Iowa 50011;Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, B-9052 Ghent, Belgium (H.N., D.I.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (H.N., D.I.); andDepartment of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071-2000 (A.L., A.W.S.).
Philip W BecraftGenetics, Development, and Cell Biology Department (G.K., H.Y., A.K.N., A.S.K., Y.Y., P.W.B.), Interdepartmental Genetics and Genomics Program (G.K., H.Y., A.S.K., Y.Y., P.W.B.), and Agronomy Department (P.W.B.), Iowa State University, Ames, Iowa 50011;Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, B-9052 Ghent, Belgium (H.N., D.I.);Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052 Ghent, Belgium (H.N., D.I.); andDepartment of Molecular Biology, University of Wyoming, Laramie, Wyoming 82071-2000 (A.L., A.W.S.) becraft@iastate.edu.ORCID http://orcid.org/0000-0002-3299-2126
Iowa State University · USUniversity of Wyoming · USVlaams Instituut voor Biotechnologie · BE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Brassinosteroids (BRs) are plant hormones involved in various growth and developmental processes. The BR signaling system is well established in Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa) but poorly understood in maize (Zea mays). BRASSINOSTEROID INSENSITIVE1 (BRI1) is a BR receptor, and database searches and additional genomic sequencing identified five maize homologs including duplicate copies of BRI1 itself. RNA interference (RNAi) using the extracellular coding region of a maize zmbri1 complementary DNA knocked down the expression of all five homologs. Decreased response to exogenously applied brassinolide and altered BR marker gene expression demonstrate that zmbri1-RNAi transgenic lines have compromised BR signaling. zmbri1-RNAi plants showed dwarf stature due to shortened internodes, with upper internodes most strongly affected. Leaves of zmbri1-RNAi plants are dark green, upright, and twisted, with decreased auricle formation. Kinematic analysis showed that decreased cell division and cell elongation both contributed to the shortened leaves. A BRASSINOSTEROID INSENSITIVE1-ETHYL METHANESULFONATE-SUPPRESSOR1-yellow fluorescent protein (BES1-YFP) transgenic line was developed that showed BR-inducible BES1-YFP accumulation in the nucleus, which was decreased in zmbri1-RNAi. Expression of the BES1-YFP reporter was strong in the auricle region of developing leaves, suggesting that localized BR signaling is involved in promoting auricle development, consistent with the zmbri1-RNAi phenotype. The blade-sheath boundary disruption, shorter ligule, and disrupted auricle morphology of RNAi lines resemble KNOTTED1-LIKE HOMEOBOX (KNOX) mutants, consistent with a mechanistic connection between KNOX genes and BR signaling.

Indexed as

Gene Knockdown TechniquesRNA InterferenceSignal TransductionAmino Acid SequenceBrassinosteroidsCell DivisionMolecular Sequence DataMutationPhenotypePlant LeavesPlant ProteinsPlants, Genetically ModifiedSequence Homology, Amino AcidSteroids, HeterocyclicZea maysbrassinolideBrassinosteroidsPlant ProteinsSteroids, Heterocyclic

Identifiers

PMID26162429
PMCPMC4577388
OpenAlexW2118610735

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