ArticlePLoS genetics2015
RFX2 Is a Major Transcriptional Regulator of Spermiogenesis.
Article in PLoS genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed, 80 citations in OpenAlex.
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- CellPolaris: Transfer Learning for Gene Regulatory Network Construction to Guide Cell State Transitions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
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- Unveiling novel insights into gene expression in monkey testes through single-nucleus-based analysis.Frontiers in cell and developmental biology · 2026Article
- The evolutionary foundations of transcriptional regulation in animals.Nature reviews. Genetics · 2025Review
- TSProm: Deciphering the Genomic Context of Tissue Specificity.bioRxiv : the preprint server for biology · 2025Article
- SETD1B-mediated broad H3K4me3 controls proper temporal patterns of gene expression critical for spermatid development.Cell research · 2025Article
- TheBiomolecules · 2025Review
- Control of ciliary transcriptional programs during spermatogenesis by antagonistic transcription factors.eLife · 2025Article
- Generation and analysis of a mouse multitissue genome annotation atlas.Genome research · 2024Article
- KDM5C is a sex-biased brake against germline gene expression programs in somatic lineages.bioRxiv : the preprint server for biology · 2024Article
- A comprehensive map of copy number variations in dromedary camels based on whole genome sequence data.Scientific reports · 2024Article
- GametesOmics: A Comprehensive Multi-omics Database for Exploring the Gametogenesis in Humans and Mice.Genomics, proteomics & bioinformatics · 2024Article
- Detection of selective sweep in European wild sheep breeds.3 Biotech · 2024Article
- Primary cilia promote the differentiation of human neurons through the WNT signaling pathway.BMC biology · 2024Article
- Generation and analysis of a mouse multi-tissue genome annotation atlas.bioRxiv : the preprint server for biology · 2024Article
- Single-cell multi-omics sequencing of human spermatogenesis reveals a DNA demethylation event associated with male meiotic recombination.Nature cell biology · 2023Article
- Transcriptional Regulation of Airway Epithelial Cell Differentiation: Insights into the Notch Pathway and Beyond.International journal of molecular sciences · 2023Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
Spermatogenesis consists broadly of three phases: proliferation of diploid germ cells, meiosis, and finally extensive differentiation of the haploid cells into effective delivery vehicles for the paternal genome. Despite detailed characterization of many haploid developmental steps leading to sperm, only fragmentary information exists on the control of gene expression underlying these processes. Here we report that the RFX2 transcription factor is a master regulator of genes required for the haploid phase. A targeted mutation of Rfx2 was created in mice. Rfx2-/- mice are perfectly viable but show complete male sterility. Spermatogenesis appears to progress unperturbed through meiosis. However, haploid cells undergo a complete arrest in spermatid development just prior to spermatid elongation. Arrested cells show altered Golgi apparatus organization, leading to a deficit in the generation of a spreading acrosomal cap from proacrosomal vesicles. Arrested cells ultimately merge to form giant multinucleated cells released to the epididymis. Spermatids also completely fail to form the flagellar axoneme. RNA-Seq analysis and ChIP-Seq analysis identified 139 genes directly controlled by RFX2 during spermiogenesis. Gene ontology analysis revealed that genes required for cilium function are specifically enriched in down- and upregulated genes showing that RFX2 allows precise temporal expression of ciliary genes. Several genes required for cell adhesion and cytoskeleton remodeling are also downregulated. Comparison of RFX2-regulated genes with those controlled by other major transcriptional regulators of spermiogenesis showed that each controls independent gene sets. Altogether, these observations show that RFX2 plays a major and specific function in spermiogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.