ArticlePloS one2015
Dual Roles of the Lysine-Rich Matrix Protein (KRMP)-3 in Shell Formation of Pearl Oyster, Pinctada fucata.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 41 citations in OpenAlex.
- Quantitative Proteomic Profiling of Pinctada fucata Shell Nacre Defines a Solubility-Based Type Classification of Shell Matrix Proteins.Marine biotechnology (New York, N.Y.) · 2026Article
- Polyamines Promote Aragonite Nucleation and Generate Biomimetic Structures.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- DNA Methylation Analyses Unveil a Regulatory Landscape in the Formation of Nacre Color in Pearl OysterFrontiers in genetics · 2022Article
- PU14, a Novel Matrix Protein, Participates in Pearl Oyster, Pinctada Fucata, Shell Formation.Marine biotechnology (New York, N.Y.) · 2021Article
- The 'Shellome' of the Crocus ClamFrontiers in genetics · 2021Article
- Recombinant transgelin-like protein 1 from Mytilus shell induces formation of CaCOFEBS open bio · 2020Article
- Molecular characterization of a whirlin-like protein with biomineralization-related functions from the shell of Mytilus coruscus.PloS one · 2020Article
- Molecular Characterization of a Novel Shell Matrix Protein With PDZ Domain FromFrontiers in physiology · 2020Article
- Transcriptional regulation of the matrix protein Shematrin-2 during shell formation in pearl oyster.The Journal of biological chemistry · 2018Article
- Cloning, characterization and functional analysis of an Alveoline-like protein in the shell of Pinctada fucata.Scientific reports · 2018Article
- A Novel Matrix Protein, PfY2, Functions as a Crucial Macromolecule during Shell Formation.Scientific reports · 2017Article
- Co-Option and De Novo Gene Evolution Underlie Molluscan Shell Diversity.Molecular biology and evolution · 2017Article
- In-depth proteomic analysis of shell matrix proteins of Pinctada fucata.Scientific reports · 2015Article
- Ingestion of gastrolith mineralized matrix increases bone volume and tissue volume in mouse long bone fracture model.Journal of orthopaedicsArticle
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix proteins play important roles in shell formation. Our group firstly isolated three cDNAs encoding lysine-rich matrix protein from Pinctada fucata in 2006. However, the functions of KRMPs are not fully understood. In addition, KRMPs contain two functional domains, the basic domain and the Gly/Tyr domain respectively. Based on the modular organization, the roles of their two domains were poorly characterized. Furthermore, KRMPs were then reported in other two species, P. maxima and P. margaritifera, which indicated that KRMPs might be very important for shell formation. In this study, the characterization and function of KRMP-3 and its two functional domains were studied in vitro through purification of recombinant glutathione S-transferase tagged KRMP-3 and two KRMP-3 deletion mutants. Western blot and immunofluorescence revealed that native KRMP-3 existed in the EDTA-insoluble matrix of the prismatic layer and was located in the organic sheet and the prismatic sheath. Recombinant KRMP-3 (rKRMP-3) bound tightly to chitin and this binding capacity was duo to the Gly/Tyr-rich region. rKRMP-3 inhibited the precipitation of CaCO3, affected the crystal morphology of calcite and inhibited the growth of aragonite in vitro, which was almost entirely attributed to the lysine-rich region. The results present direct evidence of the roles of KRMP-3 in shell biomineralization. The functional rBR region was found to participate in the growth control of crystals and the rGYR region was responsible to bind to chitin.
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