ArticlePloS one2015
Co-Orientation: Quantifying Simultaneous Co-Localization and Orientational Alignment of Filaments in Light Microscopy.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 21 citations in OpenAlex.
- Global alignment and local curvature of microtubules in mouse fibroblasts are robust against perturbations of vimentin and actin.Soft matter · 2025Article
- Intercellular Communication Through Microtubular Highways.Results and problems in cell differentiation · 2024Review
- Adaptive multiorientation resolution analysis of complex filamentous network images.Bioinformatics (Oxford, England) · 2020Article
- Long non-coding RNA LASSIE regulates shear stress sensing and endothelial barrier function.Communications biology · 2020Article
- Rudhira/BCAS3 couples microtubules and intermediate filaments to promote cell migration for angiogenic remodeling.Molecular biology of the cell · 2019Article
- The Cytoskeleton-A Complex Interacting Meshwork.Cells · 2019Review
- Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Helical organization of microtubules occurs in a minority of tunneling membrane nanotubes in normal and cancer urothelial cells.Scientific reports · 2018Article
- Quantitative fibre analysis of single-molecule localization microscopy data.Scientific reports · 2018Article
- Imaging Intermediate Filaments and Microtubules with 2-dimensional Direct Stochastic Optical Reconstruction Microscopy.Journal of visualized experiments : JoVE · 2018Article
- Vimentin on the move: new developments in cell migration.F1000Research · 2018Review
- Article
- Extracting microtubule networks from superresolution single-molecule localization microscopy data.Molecular biology of the cell · 2017Article
- Vimentin Intermediate Filaments Template Microtubule Networks to Enhance Persistence in Cell Polarity and Directed Migration.Cell systems · 2016Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Co-localization analysis is a widely used tool to seek evidence for functional interactions between molecules in different color channels in microscopic images. Here we extend the basic co-localization analysis by including the orientations of the structures on which the molecules reside. We refer to the combination of co-localization of molecules and orientational alignment of the structures on which they reside as co-orientation. Because the orientation varies with the length scale at which it is evaluated, we consider this scale as a separate informative dimension in the analysis. Additionally we introduce a data driven method for testing the statistical significance of the co-orientation and provide a method for visualizing the local co-orientation strength in images. We demonstrate our methods on simulated localization microscopy data of filamentous structures, as well as experimental images of similar structures acquired with localization microscopy in different color channels. We also show that in cultured primary HUVEC endothelial cells, filaments of the intermediate filament vimentin run close to and parallel with microtubuli. In contrast, no co-orientation was found between keratin and actin filaments. Co-orientation between vimentin and tubulin was also observed in an endothelial cell line, albeit to a lesser extent, but not in 3T3 fibroblasts. These data therefore suggest that microtubuli functionally interact with the vimentin network in a cell-type specific manner.
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