ArticlePloS one2015
Corneal Regeneration by Deep Anterior Lamellar Keratoplasty (DALK) Using Decellularized Corneal Matrix.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Corneal Endothelium Regeneration with Decellularized Porcine Corneal Extracellular Matrix Scaffolds.Tissue engineering and regenerative medicine · 2025Article
- Preparation, physico-biochemical characterization, and proteomic analysis of highly transparent corneal extracellular matrices for lamellar keratoplasty and tissue-engineered cornea construction.Materials today. Bio · 2024Article
- Use of discarded corneo-scleral rims to create cornea-like tissue.Molecular biology reports · 2024Article
- Lamellar Keratoplasty Using Acellular Bioengineering Cornea (BioCorneaVetAnimals : an open access journal from MDPI · 2022Article
- Corneal xenotransplantation: Where are we standing?Progress in retinal and eye research · 2021Review
- Article
- Engineering a Corneal Stromal Equivalent Using a Novel Multilayered Fabrication Assembly Technique.Tissue engineering. Part A · 2020Article
- Development of Transparent Acellular Dermal Matrix as Tissue-Engineered Stroma Substitute for Central Lamellar Keratoplasty.Investigative ophthalmology & visual science · 2020Article
- Decellularization methods for developing porcine corneal xenografts and future perspectives.Xenotransplantation · 2019Review
- Effects of gamma radiation sterilization on the structural and biological properties of decellularized corneal xenografts.Acta biomaterialia · 2019Article
- Extracellular Matrix Induces Periodontal Ligament Reconstruction In Vivo.International journal of molecular sciences · 2019Article
- Ultrastructural analysis of the decellularized cornea after interlamellar keratoplasty and microkeratome-assisted anterior lamellar keratoplasty in a rabbit model.Scientific reports · 2016Article
- Deep anterior lamellar keratoplasty for keratoconus: Elements for success.Saudi journal of ophthalmology : official journal of the Saudi Ophthalmological SocietyArticle
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The purpose of this study is to demonstrate the feasibility of DALK using a decellularized corneal matrix obtained by HHP methodology. Porcine corneas were hydrostatically pressurized at 980 MPa at 10°C for 10 minutes to destroy the cells, followed by washing with EGM-2 medium to remove the cell debris. The HHP-treated corneas were stained with H-E to assess the efficacy of decellularization. The decellularized corneal matrix of 300 μm thickness and 6.0 mm diameter was transplanted onto a 6.0 mm diameter keratectomy wound. The time course of regeneration on the decellularized corneal matrix was evaluated by haze grading score, fluorescein staining, and immunohistochemistry. H-E staining revealed that no cell nuclei were observed in the decellularized corneal matrix. The decellularized corneal matrices were opaque immediately after transplantation, but became completely transparent after 4 months. Fluorescein staining revealed that initial migration of epithelial cells over the grafts was slow, taking 3 months to completely cover the implant. Histological sections revealed that the implanted decellularized corneal matrix was completely integrated with the receptive rabbit cornea, and keratocytes infiltrated into the decellularized corneal matrix 6 months after transplantation. No inflammatory cells such as macrophages, or neovascularization, were observed during the implantation period. The decellularized corneal matrix improved corneal transparency, and remodelled the graft after being transplanted, demonstrating that the matrix obtained by HHP was a useful graft for corneal tissue regeneration.
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