ArticlePloS one2015
5-Aminolevulinic Acid-Based Sonodynamic Therapy Induces the Apoptosis of Osteosarcoma in Mice.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- 5-Aminolaevulinic Acid-Mediated Photodynamic Therapy Combined with Tirapazamine Enhances Efficacy in Ovarian Cancer.Biomedicines · 2025Article
- The abscopal effects of sonodynamic therapy in cancer.British journal of cancer · 2025Review
- Silent Death by Sound: CInternational journal of molecular sciences · 2023Article
- Mitochondria-targeted organic sonodynamic therapy agents: concept, benefits, and future directions.Frontiers in chemistry · 2023Review
- Current status and future perspective of sonodynamic therapy for cancer.Journal of medical ultrasonics (2001) · 2022Review
- Review
- Ultrasound-triggered oxygen-loaded nanodroplets enhance and monitor cerebral damage from sonodynamic therapy.Nanotheranostics · 2022Article
- Self-Assembling Imageable Silk Hydrogels for the Focal Treatment of Osteosarcoma.Frontiers in cell and developmental biology · 2022Article
- Sonodynamic Therapy for the Treatment of Intracranial Gliomas.Journal of clinical medicine · 2021Review
- An in vitro study on sonodynamic treatment of human colon cancer cells using sinoporphyrin sodium as sonosensitizer.Biomedical engineering online · 2020Article
- Investigation of the tumoricidal effects of sonodynamic therapy in malignant glioblastoma brain tumors.Journal of neuro-oncology · 2020Article
- 5-Aminolevulinic acid tumor paint and photodynamic therapy for myxofibrosarcoma: an in vitro study.Journal of orthopaedic surgery and research · 2020Article
- MR-guided Focused Ultrasound Facilitates Sonodynamic Therapy with 5-Aminolevulinic Acid in a Rat Glioma Model.Scientific reports · 2019Article
- Adjuvant Biophysical Therapies in Osteosarcoma.Cancers · 2019Review
- Experimental Data-Mining Analyses Reveal New Roles of Low-Intensity Ultrasound in Differentiating Cell Death Regulatome in Cancer and Non-cancer Cells via Potential Modulation of Chromatin Long-Range Interactions.Frontiers in oncology · 2019Article
- Applications of Focused Ultrasound in Cerebrovascular Diseases and Brain Tumors.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2019Review
- Protoporphyrin IX-mediated sonodynamic therapy promotes autophagy in vascular smooth muscle cells.Oncology letters · 2017Article
- Artesunate Enhances the Cytotoxicity of 5-Aminolevulinic Acid-Based Sonodynamic Therapy against Mouse Mammary Tumor Cells In Vitro.Molecules (Basel, Switzerland) · 2017Article
- Antiproliferative and Apoptosis-inducing Effect of exo-Protoporphyrin IX based Sonodynamic Therapy on Human Oral Squamous Cell Carcinoma.Scientific reports · 2017Article
- Emerging Applications of Therapeutic Ultrasound in Neuro-oncology: Moving Beyond Tumor Ablation.Neurosurgery · 2016Review
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveSonodynamic therapy (SDT) is promising for treatment of cancer, but its effect on osteosarcoma is unclear. This study examined the effect of 5-Aminolevulinic Acid (5-ALA)-based SDT on the growth of implanted osteosarcoma and their potential mechanisms in vivo and in vitro.
methodsThe dose and metabolism of 5-ALA and ultrasound periods were optimized in a mouse model of induced osteosarcoma and in UMR-106 cells. The effects of ALA-SDT on the proliferation and apoptosis of UMR-106 cells and the growth of implanted osteosarcoma were examined. The levels of mitochondrial membrane potential (ΔψM), ROS production, BcL-2, Bax, p53 and caspase 3 expression in UMR-106 cells were determined.
resultsTreatment with 5-ALA for eight hours was optimal for ALA-SDT in the mouse tumor model and treatment with 2 mM 5-ALA for 6 hours and ultrasound (1.0 MHz 2.0 W/cm2) for 7 min were optimal for UMR-106 cells. SDT, but not 5-ALA, alone inhibited the growth of implanted osteosarcoma in mice (P<0.01) and reduced the viability of UMR-106 cells (p<0.05). ALA-SDT further reduced the tumor volumes and viability of UMR-106 cells (p<0.01 for both). Pre-treatment with 5-ALA significantly enhanced the SDT-mediated apoptosis (p<0.01) and morphological changes. Furthermore, ALA-SDT significantly reduced the levels of ΔψM, but increased levels of ROS in UMR-106 cells (p<0.05 or p<0.01 vs. the Control or the Ultrasound). Moreover, ALA-SDT inhibited the proliferation of osteosarcoma cells and BcL-2 expression, but increased levels of Bax, p53 and caspase 3 expression in the implanted osteosarcoma tissues (p<0.05 or p<0.01 vs. the Control or the Ultrasound).
conclusionsThe ALA-SDT significantly inhibited osteosarcoma growth in vivo and reduced UMR-106 cell survival by inducing osteosarcoma cell apoptosis through the ROS-related mitochondrial pathway.
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