ArticlePloS one2015
Duplex Real-Time RT-PCR Assays for the Detection and Typing of Epizootic Haemorrhagic Disease Virus.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Isolating the first strain of epizootic hemorrhagic disease virus serotype 2 (EHDV-2) in mainland China.Veterinary research communications · 2026Article
- Unveiling the clinical signs and pathology in red deer (Cervus elaphus) naturally infected with epizootic haemorrhagic disease virus serotype 8.Veterinary research · 2026Article
- Epizootic Haemorrhagic Disease Virus (EHDV) Infection in Red Deer (Animals : an open access journal from MDPI · 2026Article
- Bluetongue Virus Serotype 3 Follow-Up of the 2024 Outbreak in Two Belgian Zoos.Transboundary and emerging diseases · 2026Article
- First detection of bovine viral diarrhea virus using antigen capture ELISA and RT-PCR assay in cattle with respiratory and abortive issues in Morocco.Journal of advanced veterinary and animal research · 2025Article
- Influence of inoculation dose and route on EHDV-8 distribution and the induced immune response in experimentally infected cattle.Veterinary research · 2025Article
- Impact of BTV-3 Circulation in Belgium in 2024 and Current Knowledge Gaps Hindering an Evidence-Based Control Program.Viruses · 2025Article
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- Real-Time RT-PCR Assays for Typing of Epizootic Hemorrhagic Disease Virus.Methods in molecular biology (Clifton, N.J.) · 2024Article
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- Isolation of epizootic hemorrhagic disease virus serotype 7 from cattle showing fever in Japan in 2016 and improvement of a reverse transcription-polymerase chain reaction assay to detect epizootic hemorrhagic disease virus.The Journal of veterinary medical science · 2021Article
- Development of Real-Time RT-PCR Assays for Detection and Typing of Epizootic Haemorrhagic Disease Virus.Transboundary and emerging diseases · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Epizootic haemorrhagic disease virus (EHDV) may cause severe clinical episodes in some species of deer and sometimes in cattle. Laboratory diagnosis provides a basis for the design and timely implementation of disease control measures. There are seven distinct EHDV serotypes, VP2 coding segment 2 being the target for serotype specificity. This paper reports the development and validation of eight duplex real-time RT-PCR assays to simultaneously amplify the EHDV target (S9 for the pan-EHDV real-time RT-PCR assay and S2 for the serotyping assays) and endogenous control gene Beta-actin. Analytical and diagnostic sensitivity and specificity, inter- and intra-assay variation and efficiency were evaluated for each assay. All were shown to be highly specific and sensitive.
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