ArticleBMC hematology2015
A quantitative assessment of the content of hematopoietic stem cells in mouse and human endosteal-bone marrow: a simple and rapid method for the isolation of mouse central bone marrow.
Article in BMC hematology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 23 citations in OpenAlex.
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- On the carrying capacity of the bone marrow survival niche in mice.Frontiers in immunology · 2025Article
- Development of an immunodeficient mouse that allows for conditional ablation of monocytic cells.Frontiers in immunology · 2025Article
- Multistage hematopoietic stem cell regulation in the mouse: A combined biological and mathematical approach.iScience · 2021Article
- Cathepsin K maintains the compartment of bone marrow T lymphocytes in vivo.Immunity, inflammation and disease · 2021Article
- Effect of immunosuppressants on the parasite load developed in, and immune response to, visceral leishmaniasis: A comparative study in a mouse model.PLoS neglected tropical diseases · 2021Article
- Spatiotemporal restriction of endothelial cell calcium signaling is required during leukocyte transmigration.The Journal of experimental medicine · 2021Article
- Effects of signaling pathway inhibitors on hematopoietic stem cells.Molecular medicine reports · 2021Article
- A novel approach to genetic engineering of T-cell subsets by hematopoietic stem cell infection with a bicistronic lentivirus.Scientific reports · 2020Article
- A small allelic variant in donor class I MHC is sufficient to induce alloantibodies following transfusion of standard or pathogen-reduced platelets in mice.Vox sanguinis · 2020Article
- Transient Existence of Circulating Mesenchymal Stem Cells in the Deep Veins in Humans Following Long Bone Intramedullary Reaming.Journal of clinical medicine · 2020Article
- Endothelial IQGAP1 regulates leukocyte transmigration by directing the LBRC to the site of diapedesis.The Journal of experimental medicine · 2019Article
- Bone Marrow CD133Cell transplantation · 2018Article
- Human fetal liver cultures support multiple cell lineages that can engraft immunodeficient mice.Open biology · 2017Article
- Bioluminescence Imaging Enhances Analysis of Drug Responses in a Patient-Derived Xenograft Model of Pediatric ALL.Clinical cancer research : an official journal of the American Association for Cancer Research · 2017Article
- Comparison of Human Hematopoietic Reconstitution in Different Strains of Immunodeficient Mice.Stem cells and development · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
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Abstract
backgroundIsolation of bone marrow cells, including hematopoietic stem cells, is a commonly used technique in both the research and clinical settings. A quantitative and qualitative assessment of cell populations isolated from mouse and human bone marrow was undertaken with a focus on the distribution of hematopoietic cells between the central bone marrow (cBM) and endosteal bone marrow (eBM).
methodsTwo approaches to cBM isolation from the hind legs were compared using the C57BL/6J and BALB/cJ strains of laboratory mice. The content of hematopoietic stem cells in eBM was compared to cBM from mice and human fetal bone marrow using flow cytometry. Enzymatic digestion was used to isolate eBM and its effects on antigen expression was evaluated using flow cytometry. Humanized immunodeficient mice were used to evaluate the engraftment of human precursors in the cBM and eBM and the effects of in vivo maturation on the fetal stem cell phenotype were determined.
resultsThe two methods of mouse cBM isolation yielded similar numbers of cells from the femur, but the faster single-cut method recovered more cells from the tibia. Isolation of eBM increased the yield of mouse and human stem cells. Enzymatic digestion used to isolate eBM did, however, have a detrimental effect on detecting the expression of the human HSC-antigens CD4, CD90 and CD93, whereas CD34, CD38, CD133 and HLA-DR were unaffected. Human fetal HSCs were capable of engrafting the eBM of immunodeficient mice and their pattern of CD13, CD33 and HLA-DR expression partially changed to an adult pattern of expression about 1 year after transplantation.
conclusionsA simple, rapid and efficient method for the isolation of cBM from the femora and tibiae of mice is detailed. Harvest of tibial cBM yielded about half as many cells as from the femora, representing 6.4 % and 13 %, respectively, of the total cBM of a mouse based on our analysis and a review of the literature. HSC populations were enriched within the eBM and the yield of HSCs from the mouse and human long bones was increased notably by harvest of eBM.
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