ArticleScientific reports2015
Nanophotonic detection of freely interacting molecules on a single influenza virus.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
12 citing papers in PubMed.
- Optofluidic Tweezers: Efficient and Versatile Micro/Nano-Manipulation Tools.Micromachines · 2023Review
- A Review on Photonic Sensing Technologies: Status and Outlook.Biosensors · 2023Review
- On-Chip Optical Detection of Viruses: A Review.Advanced photonics research · 2021Article
- An integrated system of air sampling and simultaneous enrichment for rapid biosensing of airborne coronavirus and influenza virus.Biosensors & bioelectronics · 2020Article
- Towards biological applications of nanophotonic tweezers.Current opinion in chemical biology · 2019Review
- Microfluidic-based high-throughput optical trapping of nanoparticles.Lab on a chip · 2017Article
- Simultaneous Characterization of Nanoparticle Size and Particle-Surface Interactions with Three-Dimensional Nanophotonic Force Microscopy.Physical review applied · 2016Article
- Label-free detection and manipulation of single biological nanoparticles.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2016Review
- Advances in biosensing strategies for HIV-1 detection, diagnosis, and therapeutic monitoring.Advanced drug delivery reviews · 2016Review
- Dynamics of an optically confined nanoparticle diffusing normal to a surface.Physical review. E · 2016Article
- Using optical trap to measure the refractive index of a single animal virus in culture fluid with high precision.Biomedical optics express · 2016Article
- Nanophotonic force microscopy: characterizing particle-surface interactions using near-field photonics.Nano letters · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Biomolecular interactions, such as antibody-antigen binding, are fundamental to many biological processes. At present, most techniques for analyzing these interactions require immobilizing one or both of the interacting molecules on an assay plate or a sensor surface. This is convenient experimentally but can constrain the natural binding affinity and capacity of the molecules, resulting in data that can deviate from the natural free-solution behavior. Here we demonstrate a label-free method for analyzing free-solution interactions between a single influenza virus and specific antibodies at the single particle level using near-field optical trapping and light-scattering techniques. We determine the number of specific antibodies binding to an optically trapped influenza virus by analyzing the change of the Brownian fluctuations of the virus. We develop an analytical model that determines the increased size of the virus resulting from antibodies binding to the virus membrane with uncertainty of ± 1-2 nm. We present stoichiometric results of 26 ± 4 (6.8 ± 1.1 attogram) anti-influenza antibodies binding to an H1N1 influenza virus. Our technique can be applied to a wide range of molecular interactions because the nanophotonic tweezer can handle molecules from tens to thousands of nanometers in diameter.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.