ArticleNature protocols2015
High-throughput microfluidics to control and measure signaling dynamics in single yeast cells.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed.
- Glucose repression ofbioRxiv : the preprint server for biology · 2026Article
- Article
- Quantifying the nuclear localization of fluorescently tagged proteins.Bioinformatics advances · 2025Article
- Enhanced cellular longevity arising from environmental fluctuations.Cell systems · 2024Article
- A fast method to distinguish between fermentative and respiratory metabolisms in single yeast cells.iScience · 2024Article
- A guide to ERK dynamics, part 2: downstream decoding.The Biochemical journal · 2023Article
- Determining growth rates from bright-field images of budding cells through identifying overlaps.eLife · 2023Article
- Using the AKAR3-EV biosensor to assess Sch9p- and PKA-signalling in budding yeast.FEMS yeast research · 2023Article
- Microfluidic dose-response platform to track the dynamics of drug response in single mycobacterial cells.Scientific reports · 2022Article
- Fungi-on-a-Chip: microfluidic platforms for single-cell studies on fungi.FEMS microbiology reviews · 2022Review
- pH dependencies of glycolytic enzymes of yeast under in vivo-like assay conditions.The FEBS journal · 2022Article
- A yeast FRET biosensor enlightens cAMP signaling.Molecular biology of the cell · 2021Article
- Challenges of analysing stochastic gene expression in bacteria using single-cell time-lapse experiments.Essays in biochemistry · 2021Article
- Promoters adopt distinct dynamic manifestations depending on transcription factor context.Molecular systems biology · 2021Article
- Regulation of the early stages of endoplasmic reticulum inheritance during ER stress.Molecular biology of the cell · 2021Article
- Paralogs in the PKA Regulon Traveled Different Evolutionary Routes to Divergent Expression in Budding Yeast.Frontiers in fungal biology · 2021Article
- Tackling microbial threats in agriculture with integrative imaging and computational approaches.Computational and structural biotechnology journal · 2021Review
- Three-dimensional localization microscopy in live flowing cells.Nature nanotechnology · 2020Article
- A protein kinase A-regulated network encodes short- and long-lived cellular memories.Science signaling · 2020Article
- An Improved ATP FRET Sensor For Yeast Shows Heterogeneity During Nutrient Transitions.ACS sensors · 2020Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Microfluidics coupled to quantitative time-lapse fluorescence microscopy is transforming our ability to control, measure and understand signaling dynamics in single living cells. Here we describe a pipeline that incorporates multiplexed microfluidic cell culture, automated programmable fluid handling for cell perturbation, quantitative time-lapse microscopy and computational analysis of time-lapse movies. We illustrate how this setup can be used to control the nuclear localization of the budding yeast transcription factor Msn2. By using this protocol, we generate oscillations of Msn2 localization and measure the dynamic gene expression response of individual genes in single cells. The protocol allows a single researcher to perform up to 20 different experiments in a single day, while collecting data for thousands of single cells. Compared with other protocols, the present protocol is relatively easy to adopt and of higher throughput. The protocol can be widely used to control and monitor single-cell signaling dynamics in other signal transduction systems in microorganisms.
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Registered trials
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