ArticleNature protocols2015
In vitro and in vivo generation and characterization of Pseudomonas aeruginosa biofilm-dispersed cells via c-di-GMP manipulation.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Low levels of the second messenger c-di-GMP enhance tolerance and resistance to meropenem inFrontiers in cellular and infection microbiology · 2026Article
- Disruption-induced changes in syntrophic propionate and acetate oxidation: flocculation, cell proximity, and microbial activity.Biotechnology for biofuels and bioproducts · 2025Article
- Antibiotic-ResistantMicroorganisms · 2025Review
- A WYL domain transcription factor regulates Lactiplantibacillus plantarum intestinal colonization via perceiving c-di-GMP.Nature communications · 2025Article
- Effect of daphnetin combined with tobramycin onFrontiers in immunology · 2025Article
- Bacterial c-di-GMP triggers metamorphosis of mussel larvae through a STING receptor.NPJ biofilms and microbiomes · 2024Article
- Hierarchical encapsulation of bacteria in functional hydrogel beads for inter- and intra- species communication.Acta biomaterialia · 2023Article
- The Alginate and Motility Regulator AmrZ is Essential for the Regulation of the Dispersion Response bymSphere · 2022Article
- On the Use of Polymer-Based Composites for the Creation of Optical Sensors: A Review.Polymers · 2022Review
- Fluorescent nanosensors reveal dynamic pH gradients during biofilm formation.NPJ biofilms and microbiomes · 2021Article
- Two-Component Signaling Systems Regulate Diverse Virulence-Associated Traits in Pseudomonas aeruginosa.Applied and environmental microbiology · 2021Article
- Approaches to Targeting Bacterial Biofilms in Cystic Fibrosis Airways.International journal of molecular sciences · 2021Review
- Mucin Glycans Signal through the Sensor Kinase RetS to Inhibit Virulence-Associated Traits in Pseudomonas aeruginosa.Current biology : CB · 2021Article
- Biofilm Formation byFrontiers in cellular and infection microbiology · 2021Article
- Systematic Analysis of c-di-GMP Signaling Mechanisms and Biological Functions in Dickeya zeae EC1.mBio · 2020Article
- Review
- Biofilm dispersion.Nature reviews. Microbiology · 2020Review
- Relief of Biofilm Hypoxia Using an Oxygen Nanocarrier: A New Paradigm for Enhanced Antibiotic Therapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
- Activity of Cinnamaldehyde on Quorum Sensing and Biofilm Susceptibility to Antibiotics inMicroorganisms · 2020Article
- Surfing motility is a complex adaptation dependent on the stringent stress response in Pseudomonas aeruginosa LESB58.PLoS pathogens · 2020Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bis-(3'-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) is a global secondary bacterial messenger that controls the formation of drug-resistant multicellular biofilms. Lowering the intracellular c-di-GMP content can disperse biofilms, and it is proposed as a biofilm eradication strategy. However, freshly dispersed biofilm cells exhibit a physiology distinct from biofilm and planktonic cells, and they might have a clinically relevant role in infections. Here we present in vitro and in vivo protocols for the generation and characterization of dispersed cells from Pseudomonas aeruginosa biofilms by reducing the intracellular c-di-GMP content through modulation of phosphodiesterases (PDEs). Unlike conventional protocols that demonstrate biofilm dispersal by biomass quantification, our protocols enable physiological characterization of the dispersed cells. Biomarkers of dispersed cells are identified and quantified, serving as potential targets for treating the dispersed cells. The in vitro protocol can be completed within 4 d, whereas the in vivo protocol requires 7 d.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.