ArticleNeurophotonics2015
Linker length and fusion site composition improve the optical signal of genetically encoded fluorescent voltage sensors.
Article in Neurophotonics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight.Neurophotonics · 2024Review
- Conserved Amino Acids Residing Outside the Voltage Field Can Shift the Voltage Sensitivity and Increase the Signal Speed and Size ofFrontiers in cell and developmental biology · 2022Article
- Mechanism of ArcLight derived GEVIs involves electrostatic interactions that can affect proton wires.Biophysical journal · 2021Article
- Red Fluorescent Genetically Encoded Voltage Indicators with Millisecond Responsiveness.Sensors (Basel, Switzerland) · 2019Article
- Engineering Photoactivatability in Genetically Encoded Voltage and pH Indicators.Frontiers in cellular neuroscience · 2019Article
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- A dimeric fluorescent protein yields a bright, red-shifted GEVI capable of population signals in brain slice.Scientific reports · 2018Article
- Voltage and Calcium Imaging of Brain Activity.Biophysical journal · 2017Review
- Modulating the Voltage-sensitivity of a Genetically Encoded Voltage Indicator.Experimental neurobiology · 2017Article
- Improving a genetically encoded voltage indicator by modifying the cytoplasmic charge composition.Scientific reports · 2017Article
- Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight.ACS chemical neuroscience · 2017Article
- A Critical and Comparative Review of Fluorescent Tools for Live-Cell Imaging.Annual review of physiology · 2017Review
- Genetically Encoded Voltage Indicators: Opportunities and Challenges.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Review
- Genetically encoded indicators of neuronal activity.Nature neuroscience · 2016Review
- Toward Better Genetically Encoded Sensors of Membrane Potential.Trends in neurosciences · 2016Review
- Pado, a fluorescent protein with proton channel activity can optically monitor membrane potential, intracellular pH, and map gap junctions.Scientific reports · 2016Article
- Imaging Membrane Potential with Two Types of Genetically Encoded Fluorescent Voltage Sensors.Journal of visualized experiments : JoVE · 2016Article
- All-optical mapping of barrel cortex circuits based on simultaneous voltage-sensitive dye imaging and channelrhodopsin-mediated photostimulation.Neurophotonics · 2015Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several genetically encoded fluorescent sensors of voltage were created by systematically truncating the length of the linker sequence between the voltage-sensing domain and the position of the fluorescent protein, Super Ecliptic A227D. In addition to varying the length, the amino acid composition at the fusion site for the fluorescent protein was modified. Both linker length and amino acid composition affected the size and voltage sensitivity of the optical signal. The truncation mutants revealed a potential structural periodicity with a maximum signal three amino acids from the voltage-sensing domain and another maximum 11 amino acids from the voltage-sensing domain. These results confirm that the linker length and composition can fine tune the size and voltage range of the sensor. The potential periodicity suggests that the orientation of the fluorescent protein could be important for improving the signal size implicating dimerization of the fluorescent protein.
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Registered trials
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