ArticleNeurophotonics2015
Using simultaneous voltage and calcium imaging to study fast Ca(2+) channels.
Article in Neurophotonics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Combining membrane potential and calcium imaging in brain slices using the voltage-sensitive dye ElectroFluor630 and the calcium indicator Calbryte520.Neurophotonics · 2026Article
- Neuronal Imaging at 8-Bit Depth to Combine High Spatial and High Temporal Resolution With Acquisition Rates Up To 40 kHz.Journal of biophotonics · 2025Article
- Fast Neuronal Calcium Signals in Brain Slices Loaded With Fluo-4 AM Ester.The European journal of neuroscience · 2025Article
- Spatial and temporal aspects of neuronal calcium and sodium signals measured with low-affinity fluorescent indicators.Pflugers Archiv : European journal of physiology · 2024Review
- Cal-520FF is the Present Optimal CaJournal of fluorescence · 2021Article
- The Origin of Physiological Local mGluR1 Supralinear CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Two Distinct Sets of CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Functional coupling of diverse voltage-gated Ca(2+) channels underlies high fidelity of fast dendritic Ca(2+) signals during burst firing.The Journal of physiology · 2016Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The combination of fluorescence measurements of membrane potential and intracellular [Formula: see text] concentration allows correlating the electrical and calcium activity of a cell with spatial precision. The technical advances allowing this type of measurement were achieved only recently and represent an important step in the progress of the voltage imaging approach pioneered over 40 years ago by Lawrence B. Cohen. Here, we show how this approach can be used to investigate the function of [Formula: see text] channels using the foreseen possibility to extract [Formula: see text] currents from imaging experiments. The kinetics of the [Formula: see text] current, mediated by voltage-gated [Formula: see text] channels, can be accurately derived from the [Formula: see text] fluorescence measurement using [Formula: see text] indicators with [Formula: see text] that equilibrate in [Formula: see text]. In this respect, the imaging apparatus dedicated to this application is described in detail. Next, we illustrate the mathematical procedure to extract the current from the [Formula: see text] fluorescence change, including a method to calibrate the signal to charge flux density. Finally, we show an example of simultaneous membrane potential and [Formula: see text] optical measurement associated with an action potential at a CA1 hippocampal pyramidal neuron from a mouse brain slice. The advantages and limitations of this approach are discussed.
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