ArticleNeurophotonics2015
Computer-generated holography enhances voltage dye fluorescence discrimination in adjacent neuronal structures.
Article in Neurophotonics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 31 citations in OpenAlex.
- Methods, Trade-offs, and Opportunities in High-speed Optical Microscopy for Neural Voltage imaging.Nature photonics · 2026Article
- Light-field deep learning enables high-throughput, scattering-mitigated calcium imaging.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Neuronal Imaging at 8-Bit Depth to Combine High Spatial and High Temporal Resolution With Acquisition Rates Up To 40 kHz.Journal of biophotonics · 2025Article
- Scanless two-photon voltage imaging.Nature communications · 2024Article
- Aberration-free holographic microscope for simultaneous imaging and stimulation of neuronal populations.Optics express · 2023Article
- DIRECT, a low-cost system for high-speed, low-noise imaging of fluorescent bio-samples.Biomedical optics express · 2023Article
- Fluorescent Reporters for Sensing Membrane Potential: Tools for Bioelectricity.Bioelectricity · 2022Article
- Spatio-temporal parameters for optical probing of neuronal activity.Biophysical reviews · 2021Review
- Genetically Encoded Voltage Indicators.Advances in experimental medicine and biology · 2021Review
- Subcellular resolution three-dimensional light-field imaging with genetically encoded voltage indicators.Neurophotonics · 2020Article
- High-Accuracy Detection of Neuronal Ensemble Activity in Two-Photon Functional Microscopy Using Smart Line Scanning.Cell reports · 2020Article
- 3D Localization for Light-Field Microscopy via Convolutional Sparse Coding on Epipolar Images.IEEE transactions on computational imaging · 2020Article
- Single-Neuron Level One-Photon Voltage Imaging With Sparsely Targeted Genetically Encoded Voltage Indicators.Frontiers in cellular neuroscience · 2019Article
- High speed functional imaging with source localized multifocal two-photon microscopy.Biomedical optics express · 2018Article
- Methods for Three-Dimensional All-Optical Manipulation of Neural Circuits.Frontiers in cellular neuroscience · 2018Review
- A Versatile and Open-Source Rapid LED Switching System for One-Photon Imaging and Photo-Activation.Frontiers in cellular neuroscience · 2018Article
- Imaging membrane potential changes from dendritic spines using computer-generated holography.Neurophotonics · 2017Article
- Vortex-free phase profiles for uniform patterning with computer-generated holography.Optics express · 2017Article
- Advances in two photon scanning and scanless microscopy technologies for functional neural circuit imaging.Proceedings of the IEEE. Institute of Electrical and Electronics Engineers · 2017Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Voltage-sensitive fluorescence indicators enable tracking neuronal electrical signals simultaneously in multiple neurons or neuronal subcompartments difficult to access with patch electrodes. However, efficient widefield epifluorescence detection of rapid voltage fluorescence transients necessitates that imaged cells and structures lie sufficiently far from other labeled structures to avoid contamination from out of focal plane and scattered light. We overcame this limitation by exciting dye fluorescence with one-photon computer-generated holography shapes contoured to axons or dendrites of interest, enabling widefield detection of voltage fluorescence with high spatial specificity. By shaping light onto neighboring axons and dendrites, we observed that dendritic back-propagating action potentials were broader and slowly rising compared with axonal action potentials, differences not measured in the same structures illuminated with a large "pseudowidefield" (pWF) spot of the same excitation density. Shaped illumination trials showed reduced baseline fluorescence, higher baseline noise, and fractional fluorescence transient amplitudes two times greater than trials acquired with pWF illumination of the same regions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.