ArticleMolecular biology of the cell2015
Disassembly activity of actin-depolymerizing factor (ADF) is associated with distinct cellular processes in apicomplexan parasites.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 22 citations in OpenAlex.
- Serum Proteinogram in the Free-Living Brazilian Common Opossum (Didelphis aurita).Electrophoresis · 2025Article
- Serum Proteomic Changes in Pet Rabbits with Subclinical and Clinical Encephalitozoonosis in Thailand.Animals : an open access journal from MDPI · 2025Article
- Recombinant actin-depolymerizing factor of the apicomplexanFrontiers in cellular and infection microbiology · 2022Article
- Differential requirements for cyclase-associated protein (CAP) in actin-dependent processes ofeLife · 2019Article
- Heterologous expression inFEBS open bio · 2018Article
- Cross-reactivity of antigens and antibodies belonging to different pathogenic types of human papillomaviruses.Doklady. Biochemistry and biophysics · 2017Article
- Article
- Intranasal immunization with recombinant Toxoplasma gondii actin depolymerizing factor confers protective efficacy against toxoplasmosis in mice.BMC immunology · 2016Article
- Comparative Plasmodium gene overexpression reveals distinct perturbation of sporozoite transmission by profilin.Molecular biology of the cell · 2016Article
- Dense granule trafficking in Toxoplasma gondii requires a unique class 27 myosin and actin filaments.Molecular biology of the cell · 2016Article
- The Actin Filament-Binding Protein Coronin Regulates Motility in Plasmodium Sporozoites.PLoS pathogens · 2016Article
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
Proteins of the actin-depolymerizing factor (ADF)/cofilin family have been shown to be crucial for the motility and survival of apicomplexan parasites. However, the mechanisms by which ADF proteins fulfill their function remain poorly understood. In this study, we investigate the comparative activities of ADF proteins from Toxoplasma gondii and Plasmodium falciparum, the human malaria parasite, using a conditional T. gondii ADF-knockout line complemented with ADF variants from either species. We show that P. falciparum ADF1 can fully restore native TgADF activity, demonstrating functional conservation between parasites. Strikingly, mutation of a key basic residue (Lys-72), previously implicated in disassembly in PfADF1, had no detectable phenotypic effect on parasite growth, motility, or development. In contrast, organelle segregation was severely impaired when complementing with a TgADF mutant lacking the corresponding residue (Lys-68). Biochemical analyses of each ADF protein confirmed the reduced ability of lysine mutants to mediate actin depolymerization via filament disassembly although not severing, in contrast to previous reports. These data suggest that actin filament disassembly is essential for apicomplexan parasite development but not for motility, as well as pointing to genus-specific coevolution between ADF proteins and their native actin.
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