Evidence map›Paper›PMID 26157148›Full record

ArticleJournal of clinical microbiology2015

Comparison of FilmArray and Quantitative Real-Time Reverse Transcriptase PCR for Detection of Zaire Ebolavirus from Contrived and Clinical Specimens.

Timothy R Southern, Lori D Racsa, César G Albariño, Paul D Fey, Steven H Hinrichs, Caitlin N Murphy, Vicki L Herrera, Anthony R Sambol, Charles E Hill, Emily L Ryan and 13 more

Registry-linked trialOpen access · bronzeAbstract readComparative StudyEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02658331 (Clinical Evaluation of the BioFire FilmArray® BioThreat-E Test for the Diagnosis of Ebola Virus Disease in West Africa), which is not on this map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
5.0field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT02658331 completednot on this map

Clinical Evaluation of the BioFire FilmArray® BioThreat-E Test for the Diagnosis of Ebola Virus Disease in West Africa

TypeobservationalSponsorBioMérieuxRan2015 to 2015Enrolled156ConditionsEbola Virus Disease
3 · Its place in the literature

Who cites it

19 citing papers in PubMed, 40 citations in OpenAlex.

  1. Article
  2. Diagnostics of Ebola virus.Frontiers in public health · 2023
    Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. The current landscape of nucleic acid tests for filovirus detection.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2018
    Review
  10. Article
  11. Review
  12. Article
  13. Review
  14. Review
  15. Article
  16. Article
  17. Article
  18. Viral Hemorrhagic Fever Diagnostics.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2016
    Review
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors at 4 institutions in 1 country.

Timothy R SouthernDepartment of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA Nebraska Public Health Laboratory, Omaha, Nebraska, USA.
Lori D RacsaDepartment of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, USA.
César G AlbariñoCenters for Disease Control and Prevention, Atlanta, Georgia, USA.
Paul D FeyDepartment of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA.
Steven H HinrichsDepartment of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA.
Caitlin N MurphyDepartment of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA Nebraska Public Health Laboratory, Omaha, Nebraska, USA.
Vicki L HerreraNebraska Public Health Laboratory, Omaha, Nebraska, USA.
Anthony R SambolNebraska Public Health Laboratory, Omaha, Nebraska, USA.
Charles E HillDepartment of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, USA.
Emily L RyanDepartment of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, USA.
Colleen S KraftDepartment of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, USA Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA.
Shelley CampbellCenters for Disease Control and Prevention, Atlanta, Georgia, USA.
Tara K SealyCenters for Disease Control and Prevention, Atlanta, Georgia, USA.
Amy SchuhCenters for Disease Control and Prevention, Atlanta, Georgia, USA.
James C RitchieDepartment of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, USA.
G Marshall LyonDepartment of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA.
Aneesh K MehtaDepartment of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA.
Jay B VarkeyDepartment of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA.
Bruce S RibnerDepartment of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA.
Kent P BrantlySamaritan's Purse, Boone, North Carolina, USA.
Ute StröherCenters for Disease Control and Prevention, Atlanta, Georgia, USA.
Peter C IwenDepartment of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, Nebraska, USA Nebraska Public Health Laboratory, Omaha, Nebraska, USA.
Eileen M BurdDepartment of Pathology and Laboratory Medicine, Emory University, Atlanta, Georgia, USA Department of Medicine, Division of Infectious Diseases, Emory University School of Medicine, Atlanta, Georgia, USA eburd@emory.edu.
Emory University · USCenters for Disease Control and Prevention · USUniversity of Nebraska Medical Center · USSmiths Detection (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Rapid, reliable, and easy-to-use diagnostic assays for detection of Zaire ebolavirus (ZEBOV) are urgently needed. The goal of this study was to examine the agreement among emergency use authorization (EUA) tests for the detection of ZEBOV nucleic acids, including the BioFire FilmArray BioThreat (BT) panel, the FilmArray BT-E panel, and the NP2 and VP40 quantitative real-time reverse transcriptase (qRT) PCR assays from the Centers for Disease Control and Prevention (CDC). Specimens used in this study included whole blood spiked with inactivated ZEBOV at known titers and whole-blood, plasma, and urine clinical specimens collected from persons diagnosed with Ebola virus disease (EVD). The agreement for FilmArray and qRT-PCR results using contrived whole-blood specimens was 100% (6/6 specimens) for each ZEBOV dilution from 4 × 10(7) to 4 × 10(2) 50% tissue culture infective dose (TCID50)/ml, as well as the no-virus negative-control sample. The limit of detection for FilmArray and qRT-PCR assays with inactivated ZEBOV, based on duplicate positive results, was determined to be 4 × 10(2) TCID50/ml. Rates of agreement between FilmArray and qRT-PCR results for clinical specimens from patients with EVD were 85% (23/27 specimens) for whole-blood specimens, 90% (18/20 specimens) for whole-blood specimens tested by FilmArray testing and matched plasma specimens tested by qRT-PCR testing, and 85% (11/13 specimens) for urine specimens. Among 60 specimens, eight discordant results were noted, with ZEBOV nucleic acids being detected only by FilmArray testing in four specimens and only by qRT-PCR testing in the remaining four specimens. These findings demonstrate that the rapid and easy-to-use FilmArray panels are effective tests for evaluating patients with EVD.

Indexed as

EbolavirusHemorrhagic Fever, EbolaHumansMolecular Diagnostic TechniquesPlasmaPolymerase Chain ReactionReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityUrine

Identifiers

PMID26157148
PMCPMC4540924
OpenAlexW1642107847

What OpenQuestion holds

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.