ArticleToxicologic pathology2015
Introduction of Zinc-salt Fixation for Effective Detection of Immune Cell-related Markers by Immunohistochemistry.
Article in Toxicologic pathology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Protocol for mapping tumor infiltration by murine T cells using multiplex immunofluorescence.STAR protocols · 2026Article
- Zinc-based fixative as a novel approach for histological preservation: A comparative study with formalin-based fixatives.Open veterinary journal · 2024Article
- Tumor microenvironmental determinants of high-risk DCIS progression.Research square · 2024Article
- CXCL9/10-engineered dendritic cells promote T cell activation and enhance immune checkpoint blockade for lung cancer.Cell reports. Medicine · 2024Article
- Immunohistochemical Characterization of Immune System Cells in Lymphoid Organs from Roe and Fallow Deer.Animals : an open access journal from MDPI · 2022Article
- Development of a Multiplex Immunohistochemistry Workflow to Investigate the Immune Microenvironment in Mouse Models of Inflammatory Bowel Disease and Colon Cancer.International journal of molecular sciences · 2021Article
- Preparation of Ex Vivo Rodent Phantoms for Developing, Testing, and Training MR Imaging of the Kidney and Other Organs.Methods in molecular biology (Clifton, N.J.) · 2021Article
- FTY720 reactivates cryptococcal granulomas in mice through S1P receptor 3 on macrophages.The Journal of clinical investigation · 2020Article
- Basal and Infectious Enteritis in Broilers Under the I See Inside Methodology: A Chronological Evaluation.Frontiers in veterinary science · 2019Article
- Tracking Modified Vaccinia Virus Ankara in the Chicken Embryo: In Vivo Tropism and Pathogenesis of Egg Infections.Viruses · 2018Article
- Toll-like receptor 3 (TLR3) promotes the resolution of Chlamydia muridarum genital tract infection in congenic C57BL/6N mice.PloS one · 2018Article
- Partial Depletion of Peripheral M1 Macrophages Reverses Motor Deficits in MPTP-Treated Mouse by Suppressing Neuroinflammation and Dopaminergic Neurodegeneration.Frontiers in aging neuroscience · 2018Article
- Approaches to Evaluate Lung Inflammation in Translational Research.Veterinary pathology · 2018Review
- Aging Mouse Models Reveal Complex Tumor-Microenvironment Interactions in Cancer Progression.Frontiers in cell and developmental biology · 2018Review
- Pathobiology of the 129:Stat1Breast cancer research : BCR · 2017Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Tissue localization of immune cells is critical to the study of disease processes in mouse models of human diseases. However, immunohistochemistry (IHC) for immune cell phenotyping in mouse tissue sections presents specific technical challenges. For example, CD4 and CD8 have been difficult to detect using IHC on formalin-fixed and paraffin-embedded mouse tissue, prompting alternative methods. We investigated the use of formalin-free zinc-salt fixation (ZN) and optimized IHC protocols for detecting a panel of immune cell-related markers (CD3, CD4, CD8, Foxp3, B220, F4/80, CD68, and major histocompatibility complex [MHC] class-I, MHC class-II, and Gr-1). The IHC results for these markers were compared on mouse spleen tissue treated with neutral buffered formalin (NBF) or ZN with or ZN without antigen retrieval (AR). Whereas CD4 and CD8 were not detected in NBF-treated tissue, all markers were detected in ZN-treated tissue without AR. Thus, the use of ZN treatment for IHC staining can be a good tool for studying immunoreactive lesions in tissues.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.