ArticleScientific reports2015
Revealing Missing Human Protein Isoforms Based on Ab Initio Prediction, RNA-seq and Proteomics.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 57 citations in OpenAlex.
- Recessive variants in the intergenic NOS1AP-C1orf226 locus cause monogenic kidney disease responsive to anti-proteinuric treatment.Nature communications · 2025Article
- Alternative splicing in shaping the molecular landscape of the cochlea.Frontiers in cell and developmental biology · 2023Review
- Individual Genetic Heterogeneity.Genes · 2022Review
- EnhancingGenes · 2022Article
- Characterization of Alternative Splicing Events in Porcine Skeletal Muscles with Different Intramuscular Fat Contents.Biomolecules · 2022Article
- A robust and stable gene selection algorithm based on graph theory and machine learning.Human genomics · 2021Article
- Assessing the functional relevance of splice isoforms.NAR genomics and bioinformatics · 2021Article
- Systematics for types and effects of RNA variations.RNA biology · 2021Article
- Analysis of Protein-Protein Interactions by Protein Microarrays.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Regulation of ADAMTS Proteases.Frontiers in molecular biosciences · 2021Review
- An analysis of tissue-specific alternative splicing at the protein level.PLoS computational biology · 2020Article
- Exploring the functional impact of alternative splicing on human protein isoforms using available annotation sources.Briefings in bioinformatics · 2019Review
- Translatomics: The Global View of Translation.International journal of molecular sciences · 2019Review
- Isoform Evolution in Primates through Independent Combination of Alternative RNA Processing Events.Molecular biology and evolution · 2017Article
- Novel promoters and coding first exons in DLG2 linked to developmental disorders and intellectual disability.Genome medicine · 2017Article
- Alternative Splicing May Not Be the Key to Proteome Complexity.Trends in biochemical sciences · 2017Review
- Diagnostic 'omics' for active tuberculosis.BMC medicine · 2016Review
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Authors and funding
15 authors at 7 institutions in 4 countries.
Funding
Abstract
Biological and biomedical research relies on comprehensive understanding of protein-coding transcripts. However, the total number of human proteins is still unknown due to the prevalence of alternative splicing. In this paper, we detected 31,566 novel transcripts with coding potential by filtering our ab initio predictions with 50 RNA-seq datasets from diverse tissues/cell lines. PCR followed by MiSeq sequencing showed that at least 84.1% of these predicted novel splice sites could be validated. In contrast to known transcripts, the expression of these novel transcripts were highly tissue-specific. Based on these novel transcripts, at least 36 novel proteins were detected from shotgun proteomics data of 41 breast samples. We also showed L1 retrotransposons have a more significant impact on the origin of new transcripts/genes than previously thought. Furthermore, we found that alternative splicing is extraordinarily widespread for genes involved in specific biological functions like protein binding, nucleoside binding, neuron projection, membrane organization and cell adhesion. In the end, the total number of human transcripts with protein-coding potential was estimated to be at least 204,950.
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