ArticleIntegrative biology : quantitative biosciences from nano to macro2015
Single cell super-resolution imaging of E. coli OmpR during environmental stress.
Article in Integrative biology : quantitative biosciences from nano to macro, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
- Expanding the microbiologist toolboxMicrobiology spectrum · 2024Article
- Visualizing the invisible: novel approaches to visualizing bacterial proteins and host-pathogen interactions.Frontiers in bioengineering and biotechnology · 2024Review
- Similarly slow diffusion of BAM and SecYEG complexes in live E. coli cells observed with 3D spt-PALM.Biophysical journal · 2023Article
- Salmonella T3SS effector SseK1 arginine-glycosylates the two-component response regulator OmpR to alter bile salt resistance.Scientific reports · 2023Article
- Bacterial Vivisection: How Fluorescence-Based Imaging Techniques Shed a Light on the Inner Workings of Bacteria.Microbiology and molecular biology reviews : MMBR · 2020Review
- Synthesis of histidine kinase inhibitors and their biological properties.Medicinal research reviews · 2020Review
- Statistical analysis of 3D localisation microscopy images for quantification of membrane protein distributions in a platelet clot model.PLoS computational biology · 2020Article
- EnvZ/OmpR Two-Component Signaling: An Archetype System That Can Function Noncanonically.EcoSal Plus · 2020Review
- Synthetic and genetic dimers as quantification ruler for single-molecule counting with PALM.Molecular biology of the cell · 2019Article
- Article
- ColiCoords: A Python package for the analysis of bacterial fluorescence microscopy data.PloS one · 2019Article
- Optimization of sample preparation and green color imaging using the mNeonGreen fluorescent protein in bacterial cells for photoactivated localization microscopy.Scientific reports · 2018Article
- Nanoporous Polymers Based on Liquid Crystals.Materials (Basel, Switzerland) · 2018Review
- A New Role of OmpR in Acid and Osmotic Stress inFrontiers in microbiology · 2018Article
- Non-canonical activation of OmpR drives acid and osmotic stress responses in single bacterial cells.Nature communications · 2017Article
- Optical and force nanoscopy in microbiology.Nature microbiology · 2016Review
- Changes in the Vibrio harveyi Cell Envelope Subproteome During Permanence in Cold Seawater.Microbial ecology · 2016Article
- Cytoplasmic sensing by the inner membrane histidine kinase EnvZ.Progress in biophysics and molecular biology · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
Abstract
Two-component signaling systems are a major strategy employed by bacteria, and to some extent, yeast and plants, to respond to environmental stress. The EnvZ/OmpR system in E. coli responds to osmotic and acid stress and is responsible for regulating the protein composition of the outer membrane. EnvZ is a histidine kinase located in the inner membrane. Upon activation, it is autophosphorylated by ATP and subsequently, it activates OmpR. Phosphorylated OmpR binds with high affinity to the regulatory regions of the ompF and ompC porin genes to regulate their transcription. We set out to visualize these two-components in single bacterial cells during different environmental stress conditions and to examine the subsequent modifications to the bacterial nucleoid as a result. We created a chromosomally-encoded, active, fluorescent OmpR-PAmCherry fusion protein and compared its expression levels with RNA polymerase. Quantitative western blotting had indicated that these two proteins were expressed at similar levels. From our images, it is evident that OmpR is significantly less abundant compared to RNA polymerase. In cross-sectional axial images, we observed OmpR molecules closely juxtaposed near the inner membrane during acidic and hyposomotic growth. In acidic conditions, the chromosome was compacted. Surprisingly, under acidic conditions, we also observed evidence of a spatial correlation between the DNA and the inner membrane, suggesting a mechanical link through an active DNA-OmpR-EnvZ complex. This work represents the first direct visualization of a response regulator with respect to the bacterial chromosome.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.