ArticleIntegrative biology : quantitative biosciences from nano to macro2015
Micropatterning of TCR and LFA-1 ligands reveals complementary effects on cytoskeleton mechanics in T cells.
Article in Integrative biology : quantitative biosciences from nano to macro, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 97 citations in OpenAlex.
- Metabolic rewiring of the dendritic cell-T cell axis: tumour-derived barriers and therapeutic opportunities.Experimental & molecular medicine · 2026Review
- LFA-1 mechanical dose controls DNAM-1 signalling and perforin polarization in NK cells.RSC advances · 2026Article
- Mechanical communication and function regulation of immune cells.Fundamental research · 2026Review
- Advances in ligand-based surface engineering strategies for fine-tuning T cell mechanotransduction toward efficient immunotherapy.Biophysical journal · 2025Review
- Article
- Mechanosensitive biochemical imprinting of the talin interaction with DLC1 regulates RhoA activity and cardiomyocyte remodeling.Science advances · 2025Article
- Immunological synapse: structures, molecular mechanisms and therapeutic implications in disease.Signal transduction and targeted therapy · 2025Review
- Platforms for studying cell-cell recognition by immune cells.Immunology and cell biology · 2025Review
- Matrix-associated extracellular vesicles modulate smooth muscle cell adhesion and directionality by presenting collagen VI.bioRxiv : the preprint server for biology · 2025Article
- Ligand-induced segregation from large cell-surface phosphatases is a critical step in γδ TCR triggering.Cell reports · 2024Article
- Morphodynamics of T-lymphocytes: Scanning to spreading.Biophysical journal · 2024Review
- Human T-cell receptor triggering requires inactivation of Lim kinase-1 by Slingshot-1 phosphatase.Communications biology · 2024Article
- Mechanical regulation of lymphocyte activation and function.Journal of cell science · 2024Review
- Review
- Mechanical forces amplify TCR mechanotransduction in T cell activation and function.Applied physics reviews · 2024Review
- Hypertensive Pressure Mechanosensing Alone Triggers Lipid Droplet Accumulation and Transdifferentiation of Vascular Smooth Muscle Cells to Foam Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Increased H3K9me3 and F-Actin Reorganization in the Rapid Adaptive Response to Hypergravity in Human T Lymphocytes.International journal of molecular sciences · 2023Article
- Mechanobiological Strategies to Augment Cancer Treatment.ACS omega · 2023Review
- T Cell engineering for cancer immunotherapy by manipulating mechanosensitive force-bearing receptors.Frontiers in bioengineering and biotechnology · 2023Review
- Regulation of cardiomyocyte adhesion and mechanosignalling through distinct nanoscale behaviour of integrin ligands mimicking healthy or fibrotic extracellular matrix.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 5 countries.
Funding
Abstract
The formation of the immunological synapse between a T cell and the antigen-presenting cell (APC) is critically dependent on actin dynamics, downstream of T cell receptor (TCR) and integrin (LFA-1) signalling. There is also accumulating evidence that mechanical forces, generated by actin polymerization and/or myosin contractility regulate T cell signalling. Because both receptor pathways are intertwined, their contributions towards the cytoskeletal organization remain elusive. Here, we identify the specific roles of TCR and LFA-1 by using a combination of micropatterning to spatially separate signalling systems and nanopillar arrays for high-precision analysis of cellular forces. We identify that Arp2/3 acts downstream of TCRs to nucleate dense actin foci but propagation of the network requires LFA-1 and the formin FHOD1. LFA-1 adhesion enhances actomyosin forces, which in turn modulate actin assembly downstream of the TCR. Together our data shows a mechanically cooperative system through which ligands presented by an APC modulate T cell activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.