ArticleScientific reports2015
Efficient introgression of allelic variants by embryo-mediated editing of the bovine genome.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 56 citations in OpenAlex.
- Versatile generation of precise gene edits in bovines using SEGCPN.BMC biology · 2023Article
- Cytoplasmic Injection of Zygotes to Genome Edit Naturally Occurring Sequence Variants Into Bovine Embryos.Frontiers in genetics · 2022Article
- Holstein Friesian dairy cattle edited for diluted coat color as a potential adaptation to climate change.BMC genomics · 2021Article
- Impact of CRISPR-Cas9-Based Genome Engineering in Farm Animals.Veterinary sciences · 2021Review
- Evaluation of mutation rates, mosaicism and off target mutations when injecting Cas9 mRNA or protein for genome editing of bovine embryos.Scientific reports · 2020Article
- One-Step Homology Mediated CRISPR-Cas Editing in Zygotes for Generating Genome Edited Cattle.The CRISPR journal · 2020Article
- Harnessing endogenous repair mechanisms for targeted gene knock-in of bovine embryos.Scientific reports · 2020Article
- Production of hypoallergenic milk from DNA-free beta-lactoglobulin (BLG) gene knockout cow using zinc-finger nucleases mRNA.Scientific reports · 2018Article
- Cattle with a precise, zygote-mediated deletion safely eliminate the major milk allergen beta-lactoglobulin.Scientific reports · 2018Article
- The impact of genome editing on the introduction of monogenic traits in livestock.Genetics, selection, evolution : GSE · 2018Article
- Development of genome engineering technologies in cattle: from random to specific.Journal of animal science and biotechnology · 2018Review
- Correction of a Disease Mutation using CRISPR/Cas9-assisted Genome Editing in Japanese Black Cattle.Scientific reports · 2017Article
- Genome editing in livestock: Are we ready for a revolution in animal breeding industry?Transgenic research · 2017Review
- Advances in genome editing for improved animal breeding: A review.Veterinary world · 2017Review
- A history of genome editing in mammals.Mammalian genome : official journal of the International Mammalian Genome Society · 2017Review
- Generation of gene edited birds in one generation using sperm transfection assisted gene editing (STAGE).Transgenic research · 2017Article
- Isozygous and selectable marker-free MSTN knockout cloned pigs generated by the combined use of CRISPR/Cas9 and Cre/LoxP.Scientific reports · 2016Article
- Gene targeting, genome editing: from Dolly to editors.Transgenic research · 2016Review
- One-step Multiplex Transgenesis via Sleeping Beauty Transposition in Cattle.Scientific reports · 2016Article
- Engineered Swine Models of Cancer.Frontiers in genetics · 2016Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recent development of designer nucleases allows for the efficient and precise introduction of genetic change into livestock genomes. Most studies so far have focused on the introduction of random mutations in cultured cells and the use of nuclear transfer to generate animals with edited genotypes. To circumvent the intrinsic uncertainties of random mutations and the inefficiencies of nuclear transfer we directed our efforts to the introduction of specific genetic changes by homology-driven repair directly in in vitro produced embryos. Initially, we injected zinc finger nuclease (ZFN)-encoding mRNA or DNA into bovine zygotes to verify cleavage activity at their target site within the gene for beta-lactoglobulin (LGB) and detected ZFN-induced random mutations in 30% to 80% of embryos. Next, to precisely change the LGB sequence, we co-injected ZFNs or transcription activator-like effector nucleases (TALENs) with DNA oligonucleotides (ODNs). Analysis of co-injected embryos showed targeted changes in up to 33% (ZFNs) and 46% (TALENs) of blastocysts. Deep sequence analysis of selected embryos revealed contributions of the targeted LGB allele can reach 100% which implies that genome editing by zygote injections can facilitate the one-step generation of non-mosaic livestock animals with pre-designed biallelic modifications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.