ArticleeLife2015
Identification of polarized macrophage subsets in zebrafish.
Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 163 papers, 1 of them a synthesis that pooled it.
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Who cites it
163 citing papers in PubMed, 1 synthesis or guideline pooled it, 300 citations in OpenAlex.
- Role of Macrophages in Cardiac Remodeling: Cues From Zebrafish Heart.Immunity, inflammation and disease · 2026Pooled it
- Review
- Novel polyvinyl alcohol-borax hydrogel with potential to accelerate skin wound healing in aquatic models: zebrafish and tilapia.Royal Society open science · 2026Article
- The microglia-derived protein Sema4ab attenuates regenerative neurogenesis after spinal cord injury in zebrafish.PLoS biology · 2026Article
- Zebrafish Embryos to Profile Nano(bio)materials: A Modular Platform for Developmental Toxicity, Neurotoxicity, and Inflammation-Regeneration Assays.Current protocols · 2026Article
- Exploring mechanisms of scar-free skin wound healing in adult zebrafish in comparison to mouse.PLoS genetics · 2026Article
- Calcium Signaling in Macrophages During a Wound Response In Vivo.International journal of molecular sciences · 2026Article
- Loss of Cathepsin Z enhances pro-inflammatory macrophage responses and promotes tissue regeneration.Disease models & mechanisms · 2026Article
- In vivo screen reveals specific roles of Hippo pathway components in development and regeneration.Life science alliance · 2026Article
- Spatial microenvironments tune immune response dynamics in the Drosophila larval fat body.PLoS genetics · 2026Article
- The morpho-kinetic landscape of macrophage modes during wound healing in zebrafish.Scientific reports · 2026Article
- Mammalian mesenchymal stromal cells enhance zebrafish fin regeneration.Cell regeneration (London, England) · 2026Article
- Chronic macrophage activation derails muscle repair by disrupting mannose-receptor-linked plasticity revealed by endogenous irg1/acod1 tracking.Nature communications · 2026Article
- Tumour-specific STING agonist synthesis via a two-component prodrug system.Nature chemistry · 2025Article
- Article
- Mechanisms underpinning spontaneous spinal cord regeneration.Development (Cambridge, England) · 2025Review
- Spatial microenvironments tune immune response dynamics in thebioRxiv : the preprint server for biology · 2025Article
- Metainflammation alters neutrophil function and migration in vivo in response to tissue injury.Journal of leukocyte biology · 2025Article
- Modeling Pseudomonas aeruginosa-Staphylococcus aureus interactions in zebrafish to assess the host inflammatory response upon co-infection.Scientific reports · 2025Article
- An NF-kB/TNF-alpha signalling feedback loop acts to coordinate tissue regeneration and macrophage behaviour in zebrafish.NPJ Regenerative medicine · 2025Article
103 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
While the mammalian macrophage phenotypes have been intensively studied in vitro, the dynamic of their phenotypic polarization has never been investigated in live vertebrates. We used the zebrafish as a live model to identify and trail macrophage subtypes. We generated a transgenic line whose macrophages expressing tumour necrosis factor alpha (tnfa), a key feature of classically activated (M1) macrophages, express fluorescent proteins Tg(mpeg1:mCherryF/tnfa:eGFP-F). Using 4D-confocal microscopy, we showed that both aseptic wounding and Escherichia coli inoculation triggered macrophage recruitment, some of which started to express tnfa. RT-qPCR on Fluorescence Activated Cell Sorting (FACS)-sorted tnfa(+) and tnfa(-) macrophages showed that they, respectively, expressed M1 and alternatively activated (M2) mammalian markers. Fate tracing of tnfa(+) macrophages during the time-course of inflammation demonstrated that pro-inflammatory macrophages converted into M2-like phenotype during the resolution step. Our results reveal the diversity and plasticity of zebrafish macrophage subsets and underline the similarities with mammalian macrophages proposing a new system to study macrophage functional dynamic.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.