ArticlePloS one2015
BcMF26a and BcMF26b Are Duplicated Polygalacturonase Genes with Divergent Expression Patterns and Functions in Pollen Development and Pollen Tube Formation in Brassica campestris.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Genome-Wide Identification of NAC Gene Family Members of Tree Peony (International journal of molecular sciences · 2024Article
- Genome-wide identification, characterization and expression analysis of the DUF668 gene family in tomato.PeerJ · 2024Article
- Genome-wide identification and expression analysis of the polygalacturonase gene family in sweetpotato.BMC plant biology · 2023Article
- Sequential Deposition and Remodeling of Cell Wall Polymers During Tomato Pollen Development.Frontiers in plant science · 2021Article
- Patterns of Expansion and Expression Divergence of the Polygalacturonase Gene Family inInternational journal of molecular sciences · 2020Article
- A Complex Journey: Cell Wall Remodeling, Interactions, and Integrity During Pollen Tube Growth.Frontiers in plant science · 2020Review
- Evolution of MIR159/319 genes in Brassica campestris and their function in pollen development.Plant molecular biology · 2019Article
- CircRNA Expression Pattern and ceRNA and miRNA-mRNA Networks Involved in Anther Development in the CMS Line ofInternational journal of molecular sciences · 2019Article
- Article
- Evolution of Cell Wall Polymers in Tip-Growing Land Plant Gametophytes: Composition, Distribution, Functional Aspects and Their Remodeling.Frontiers in plant science · 2019Review
- GCTTCA as a novel motif for regulating mesocarp-specific expression of the oil palm (Elaeis guineensis Jacq.) stearoyl-ACP desaturase gene.Plant cell reports · 2018Article
- The Arabidopsis COPII components, AtSEC23A and AtSEC23D, are essential for pollen wall development and exine patterning.Journal of experimental botany · 2018Article
- Over-expression of miR158 causes pollen abortion in Brassica campestris ssp. chinensis.Plant molecular biology · 2017Article
- Article
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polygalacturonase (PG) is one of the cell wall hydrolytic enzymes involving in pectin degradation. A comparison of two highly conserved duplicated PG genes, namely, Brassica campestris Male Fertility 26a (BcMF26a) and BcMF26b, revealed the different features of their expression patterns and functions. We found that these two genes were orthologous genes of At4g33440, and they originated from a chromosomal segmental duplication. Although structurally similar, their regulatory and intron sequences largely diverged. QRT-PCR analysis showed that the expression level of BcMF26b was higher than that of BcMF26a in almost all the tested organs and tissues in Brassica campestris. Promoter activity analysis showed that, at reproductive development stages, BcMF26b promoter was active in tapetum, pollen grains, and pistils, whereas BcMF26a promoter was only active in pistils. In the subcellular localization experiment, BcMF26a and BcMF26b proteins could be localized to the cell wall. When the two genes were co-inhibited, pollen intine was formed abnormally and pollen tubes could not grow or stretch. Moreover, the knockout mutants of At4g33440 delayed the growth of pollen tubes. Therefore, BcMF26a/b can participate in the construction of pollen wall by modulating intine information and BcMF26b may play a major role in co-inhibiting transformed plants.
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