ArticleJournal of industrial microbiology & biotechnology2015
Exploring medium-chain-length polyhydroxyalkanoates production in the engineered yeast Yarrowia lipolytica.
Article in Journal of industrial microbiology & biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 52 citations in OpenAlex.
- Marine Yeasts for Biotechnology: Potential Applications and Insights from Comparisons with Terrestrial Yeasts.Journal of fungi (Basel, Switzerland) · 2026Review
- Synthetic biology strategies for sustainable bioplastic production by yeasts.Journal of microbiology (Seoul, Korea) · 2025Review
- Uncovering novel polyhydroxyalkanoate biosynthesis genes and unique pathway in yeast hanseniaspora valbyensis for sustainable bioplastic production.Scientific reports · 2024Article
- Potato Peel Waste as an Economic Feedstock for PHA Production by Bacillus circulans.Applied biochemistry and biotechnology · 2024Article
- Evaluation of polyhydroxyalkanoate (PHA) synthesis by Pichia sp. TSLS24 yeast isolated in Vietnam.Scientific reports · 2023Article
- Control of D-lactic acid content in P(LA-3HB) copolymer in the yeastMetabolic engineering communications · 2022Article
- Review
- Production of D-lactic acid containing polyhydroxyalkanoate polymers in yeast Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2021Article
- Engineering the YeastFrontiers in bioengineering and biotechnology · 2020Article
- Production and characterization of two medium-chain-length polydroxyalkanoates by engineered strains of Yarrowia lipolytica.Microbial cell factories · 2019Article
- Synthetic biology tools for engineering Yarrowia lipolytica.Biotechnology advances · 2018Review
- Engineering Yarrowia lipolytica for Use in Biotechnological Applications: A Review of Major Achievements and Recent Innovations.Molecular biotechnology · 2018Review
- Harnessing yeast organelles for metabolic engineering.Nature chemical biology · 2017Review
- Dual-Target Bioprocessing Using Oleaginous Microorganisms: Converting Food Waste into Lipids and Biopolymers.Food technology and biotechnologyReview
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Authors and funding
4 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Medium-chain-length polyhydroxyalkanoates (mcl-PHAs) are a large class of biopolymers that have attracted extensive attention as renewable and biodegradable bio-plastics. They are naturally synthesized via fatty acid de novo biosynthesis pathway or β-oxidation pathway from Pseudomonads. The unconventional yeast Yarrowia lipolytica has excellent lipid/fatty acid catabolism and anabolism capacity depending of the mode of culture. Nevertheless, it cannot naturally synthesize PHA, as it does not express an intrinsic PHA synthase. Here, we constructed a genetically modified strain of Y. lipolytica by heterologously expressing PhaC1 gene from P. aeruginosa PAO1 with a PTS1 peroxisomal signal. When in single copy, the codon optimized PhaC1 allowed the synthesis of 0.205 % DCW of PHA after 72 h cultivation in YNBD medium containing 0.1 % oleic acid. By using a multi-copy integration strategy, PHA content increased to 2.84 % DCW when the concentration of oleic acid in YNBD was 1.0 %. Furthermore, when the recombinant yeast was grown in the medium containing triolein, PHA accumulated up to 5.0 % DCW with as high as 21.9 g/L DCW, which represented 1.11 g/L in the culture. Our results demonstrated the potential use of Y. lipolytica as a promising microbial cell factory for PHA production using food waste, which contains lipids and other essential nutrients.
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