ArticleDevelopment (Cambridge, England)2015
Inhibition of β-catenin signaling respecifies anterior-like endothelium into beating human cardiomyocytes.
Article in Development (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Multiplexed single cell transcriptomics optimizes mesodermal patterning and hemogenic endothelial output from murine embryonic stem cells.Research square · 2026Article
- Nanopatterned Cell Sheet Assembly of Biomimetic Cardiac Laminae for Modeling Structure-Function Relationships.Biomaterials research · 2026Article
- Feeder-free generation and characterization of endocardial and cardiac valve cells from human pluripotent stem cells.iScience · 2024Article
- Stacking thick perfusable human microvascular grafts enables dense vascularity and rapid integration into infarcted rat hearts.Biomaterials · 2023Article
- Dynamic chromatin organization and regulatory interactions in human endothelial cell differentiation.Stem cell reports · 2023Article
- Endothelial progenitor cells derived from embryonic stem cells prevent alveolar simplification in a murine model of bronchopulmonary dysplasia.Frontiers in cell and developmental biology · 2023Article
- Endocardial identity is established during early somitogenesis by Bmp signalling acting upstream of npas4l and etv2.Development (Cambridge, England) · 2022Article
- Cell type determination for cardiac differentiation occurs soon after seeding of human-induced pluripotent stem cells.Genome biology · 2022Article
- Opportunities and challenges in cardiac tissue engineering from an analysis of two decades of advances.Nature biomedical engineering · 2022Review
- Responsiveness to perturbations is a hallmark of transcription factors that maintain cell identity in vitro.Cell systems · 2021Article
- A comprehensive analysis of gene expression changes in a high replicate and open-source dataset of differentiating hiPSC-derived cardiomyocytes.Scientific reports · 2021Article
- WNT5A from the fetal liver vascular niche supports human fetal liver hematopoiesis.Stem cell research & therapy · 2021Article
- Wnt/β-catenin signaling regulates adipose tissue lipogenesis and adipocyte-specific loss is rigorously defended by neighboring stromal-vascular cells.Molecular metabolism · 2020Article
- From Human Pluripotent Stem Cells to 3D Cardiac Microtissues: Progress, Applications and Challenges.Bioengineering (Basel, Switzerland) · 2020Review
- Vertebrate cranial mesoderm: developmental trajectory and evolutionary origin.Cellular and molecular life sciences : CMLS · 2020Review
- Engineering anisotropic 3D tubular tissues with flexible thermoresponsive nanofabricated substrates.Biomaterials · 2020Article
- Absence of full-length dystrophin impairs normal maturation and contraction of cardiomyocytes derived from human-induced pluripotent stem cells.Cardiovascular research · 2020Article
- Epicardial cells derived from human embryonic stem cells augment cardiomyocyte-driven heart regeneration.Nature biotechnology · 2019Article
- Generation of Endothelial Cells From Human Pluripotent Stem Cells.Arteriosclerosis, thrombosis, and vascular biology · 2019Review
- Fluorescent Gene Tagging of Transcriptionally Silent Genes in hiPSCs.Stem cell reports · 2019Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
During vertebrate development, mesodermal fate choices are regulated by interactions between morphogens such as activin/nodal, BMPs and Wnt/β-catenin that define anterior-posterior patterning and specify downstream derivatives including cardiomyocyte, endothelial and hematopoietic cells. We used human embryonic stem cells to explore how these pathways control mesodermal fate choices in vitro. Varying doses of activin A and BMP4 to mimic cytokine gradient polarization in the anterior-posterior axis of the embryo led to differential activity of Wnt/β-catenin signaling and specified distinct anterior-like (high activin/low BMP) and posterior-like (low activin/high BMP) mesodermal populations. Cardiogenic mesoderm was generated under conditions specifying anterior-like mesoderm, whereas blood-forming endothelium was generated from posterior-like mesoderm, and vessel-forming CD31(+) endothelial cells were generated from all mesoderm origins. Surprisingly, inhibition of β-catenin signaling led to the highly efficient respecification of anterior-like endothelium into beating cardiomyocytes. Cardiac respecification was not observed in posterior-derived endothelial cells. Thus, activin/BMP gradients specify distinct mesodermal subpopulations that generate cell derivatives with unique angiogenic, hemogenic and cardiogenic properties that should be useful for understanding embryogenesis and developing therapeutics.
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