Evidence map›Paper›PMID 26152517›Full record

ArticleScientific reports2015

RalB regulates contractility-driven cancer dissemination upon TGFβ stimulation via the RhoGEF GEF-H1.

Marco Biondini, Guillaume Duclos, Nathalie Meyer-Schaller, Pascal Silberzan, Jacques Camonis, Maria Carla Parrini

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 1 pooled it
2.4field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.

  1. Physical Forces in Glioblastoma Migration: A Systematic Review.International journal of molecular sciences · 2022
    Pooled it
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  12. A Tale of Two States: Normal and Transformed, With and Without Rigidity Sensing.Annual review of cell and developmental biology · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 2 countries.

Marco Biondini1] Institut Curie, Centre de Recherche, Paris Sciences et Lettres University, 75248 Paris, France [2] ART group, Inserm U830.
Guillaume Duclos1] Institut Curie, Centre de Recherche, Paris Sciences et Lettres University, 75248 Paris, France [2] UMR168, CNRS, UPMC Equipe labellisée Ligue contre le Cancer.
Nathalie Meyer-SchallerDepartment of Biomedicine, University of Basel, Switzerland.
Pascal Silberzan1] Institut Curie, Centre de Recherche, Paris Sciences et Lettres University, 75248 Paris, France [2] UMR168, CNRS, UPMC Equipe labellisée Ligue contre le Cancer.
Jacques Camonis1] Institut Curie, Centre de Recherche, Paris Sciences et Lettres University, 75248 Paris, France [2] ART group, Inserm U830.
Maria Carla Parrini1] Institut Curie, Centre de Recherche, Paris Sciences et Lettres University, 75248 Paris, France [2] ART group, Inserm U830.
Inserm · FRInstitut Curie · FRPhysique des Cellules et Cancers · FRUniversity of Basel · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

RalA and RalB proteins are key mediators of oncogenic Ras signaling in human oncogenesis. Herein we investigated the mechanistic contribution of Ral proteins to invasion of lung cancer A549 cells after induction of epithelial-mesenchymal transition (EMT) with TGFβ. We show that TGFβ-induced EMT promotes dissemination of A549 cells in a 2/3D assay, independently of proteolysis, by activating the Rho/ROCK pathway which generates actomyosin-dependent contractility forces that actively remodel the extracellular matrix, as assessed by Traction Force microscopy. RalB, but not RalA, is required for matrix deformation and cell dissemination acting via the RhoGEF GEF-H1, which associates with the Exocyst complex, a major Ral effector. Indeed, uncoupling of the Exocyst subunit Sec5 from GEF-H1 impairs RhoA activation, generation of traction forces and cell dissemination. These results provide a novel molecular mechanism underlying the control of cell invasion by RalB via a cross-talk with the Rho pathway.

Indexed as

AmidesCell Line, TumorEpithelial-Mesenchymal TransitionHumansMicroscopy, Atomic ForcePyridinesrac1 GTP-Binding Proteinral GTP-Binding Proteinsrho-Associated KinasesRho Guanine Nucleotide Exchange FactorsRNA InterferenceRNA, Small InterferingSignal TransductionTransforming Growth Factor betaAmidesARHGEF2 protein, humanPyridinesrac1 GTP-Binding ProteinRAC1 protein, humanRALA protein, humanRalb protein, humanral GTP-Binding Proteinsrho-Associated KinasesRho Guanine Nucleotide Exchange FactorsRNA, Small InterferingTransforming Growth Factor betaY 27632

Identifiers

PMID26152517
PMCPMC4495419
OpenAlexW802380919

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.