Trial reportThe Journal of allergy and clinical immunology2016
Single B-cell deconvolution of peanut-specific antibody responses in allergic patients.
Trial report in The Journal of allergy and clinical immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
69 citing papers in PubMed, 136 citations in OpenAlex.
- Codfish Oral Immunotherapy in Children Aged 2-10: Randomized Placebo-Controlled Study.Allergy · 2026Trial
- High-resolution epitope mapping by AllerScan reveals relationships between IgE and IgG repertoires during peanut oral immunotherapy.Cell reports. Medicine · 2021Trial
- Allergen-specific IgGThe Journal of allergy and clinical immunology · 2020Trial
- Characterization of the B-cell receptor repertoires in peanut allergic subjects undergoing oral immunotherapy.Journal of human genetics · 2018Trial
- Monitoring of dynamic changes in Keyhole Limpet Hemocyanin (KLH)-specific B cells in KLH-vaccinated cancer patients.Scientific reports · 2017Trial
- Multiple roads to IgE memory: A pluralistic model of IgE immunity.Cell reports · 2026Review
- Mechanisms of IgE-mediated food allergy and the role of allergen-specific B cells.FEBS letters · 2026Review
- Allergen-specific human IgE isolated through an allergen-agnostic pipeline-understanding immune response and allergen recognition.Communications biology · 2026Article
- Allergic Reactivity and Memory Occur Independently of Sequential Switching Through IgG1.Allergy · 2025Article
- Advances in Food Allergy Immunotherapy: Current Strategies and Role of Antibodies Isotypes.Cells · 2025Review
- Germline-encoded recognition of peanut underlies development of convergent antibodies in humans.Science translational medicine · 2025Article
- Article
- Origins and diversity of pan-isotype human bone marrow plasma cells.bioRxiv : the preprint server for biology · 2024Article
- Precision engineering for localization, validation, and modification of allergenic epitopes.The Journal of allergy and clinical immunology · 2024Review
- CD23Science translational medicine · 2024Article
- Linear epitopes of bony fish β-parvalbumins.Frontiers in immunology · 2024Article
- Design of an Ara h 2 hypoallergen from conformational epitopes.Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology · 2024Article
- Clonal evolution and stereotyped sequences of human IgE lineages in aeroallergen-specific immunotherapy.The Journal of allergy and clinical immunology · 2023Article
- ViCloD, an interactive web tool for visualizing B cell repertoires and analyzing intraclonal diversities: application to human B-cell tumors.NAR genomics and bioinformatics · 2023Article
- Mechanisms of Allergen Immunotherapy and Potential Biomarkers for Clinical Evaluation.Journal of personalized medicine · 2023Review
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe frequencies, cellular phenotypes, epitope specificity, and clonal diversity of allergen-specific B cells in patients with food allergy are not fully understood but are of major pathogenic and therapeutic significance.
objectiveWe sought to characterize peanut allergen-specific B-cell populations and the sequences and binding activities of their antibodies before and during immunotherapy.
methodsB cells binding fluorescently labeled Ara h 1 or Ara h 2 were phenotyped and isolated by means of flow cytometric sorting from 18 patients at baseline and 13 patients during therapy. Fifty-seven mAbs derived from allergen-binding single B cells were evaluated by using ELISA, Western blotting, and peptide epitope mapping. Deep sequencing of the B-cell repertoires identified additional members of the allergen-specific B-cell clones.
resultsMedian allergen-binding B-cell frequencies were 0.0097% (Ara h 1) or 0.029% (Ara h 2) of B cells in baseline blood from allergic patients and approximately 3-fold higher during immunotherapy. Five of 57 allergen-specific cells belonged to clones containing IgE-expressing members. Almost all allergen-specific antibodies were mutated, and binding to both conformational and linear allergen epitopes was detected. Increasing somatic mutation of IgG4 members of a clone was seen in immunotherapy, whereas IgE mutation levels in the clone did not increase.
conclusionMost peanut allergen-binding B cells isolated by means of antigen-specific flow sorting express mutated and isotype-switched antibodies. Immunotherapy increases their frequency in the blood, and even narrowly defined allergen epitopes are recognized by numerous distinct B-cell clones in a patient. The results also suggest that oral immunotherapy can stimulate somatic mutation of allergen-specific IgG4.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.