ArticleNucleic acids research2015
Different motif requirements for the localization zipcode element of β-actin mRNA binding by HuD and ZBP1.
Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- Article
- DeepLocRNA: an interpretable deep learning model for predicting RNA subcellular localization with domain-specific transfer-learning.Bioinformatics (Oxford, England) · 2024Article
- Zbp1 gene: a modulator of multiple aging hallmarks as potential therapeutic target for age-related diseases.Biogerontology · 2023Review
- Members of the CUGBP Elav-like family of RNA-binding proteins are expressed in distinct populations of primary sensory neurons.The Journal of comparative neurology · 2023Article
- Genome-wide translation control analysis of developing human neurons.Molecular brain · 2022Article
- Review
- Coupling axonal mRNA transport and local translation to organelle maintenance and function.Current opinion in cell biology · 2022Review
- A choreography of centrosomal mRNAs reveals a conserved localization mechanism involving active polysome transport.Nature communications · 2021Article
- CircRNAs as promising biomarkers of inflammatory bowel disease and its associated-colorectal cancer.American journal of translational research · 2021Article
- Molecular mechanisms behind mRNA localization in axons.Open biology · 2020Review
- Intra-axonal mechanisms driving axon regeneration.Brain research · 2020Review
- hnRNPs Interacting with mRNA Localization Motifs Define Axonal RNA Regulons.Molecular & cellular proteomics : MCP · 2018Article
- Axonal mRNA transport and translation at a glance.Journal of cell science · 2018Review
- RNA Degradation in Neurodegenerative Disease.Advances in neurobiology · 2018Review
- Comparative Visualization of the RNA Suboptimal Conformational Ensemble In Vivo.Biophysical journal · 2017Article
- Diverse role of survival motor neuron protein.Biochimica et biophysica acta. Gene regulatory mechanisms · 2017Review
- Nucleolin-Mediated RNA Localization Regulates Neuron Growth and Cycling Cell Size.Cell reports · 2016Article
- RNA-binding proteins, neural development and the addictions.Genes, brain, and behavior · 2016Review
- Competing Interactions of RNA-Binding Proteins, MicroRNAs, and Their Targets Control Neuronal Development and Function.Biomolecules · 2015Review
- Smaug variants in neural and non-neuronal cells.Communicative & integrative biologyArticle
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Interactions of RNA-binding proteins (RBPs) with their target transcripts are essential for regulating gene expression at the posttranscriptional level including mRNA export/localization, stability, and translation. ZBP1 and HuD are RBPs that play pivotal roles in mRNA transport and local translational control in neuronal processes. While HuD possesses three RNA recognition motifs (RRMs), ZBP1 contains two RRMs and four K homology (KH) domains that either increase target specificity or provide a multi-target binding capability. Here we used isolated cis-element sequences of the target mRNA to examine directly protein-RNA interactions in cell-free systems. We found that both ZBP1 and HuD bind the zipcode element in rat β-actin mRNA's 3' UTR. Differences between HuD and ZBP1 were observed in their binding preference to the element. HuD showed a binding preference for U-rich sequence. In contrast, ZBP1 binding to the zipcode RNA depended more on the structural level, as it required the proper spatial organization of a stem-loop that is mainly determined by the U-rich element juxtaposed to the 3' end of a 5'-ACACCC-3' motif. On the basis of this work, we propose that ZBP1 and HuD bind to overlapping sites in the β-actin zipcode, but they recognize different features of this target sequence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.