ArticleACS chemical biology2015
eZinCh-2: A Versatile, Genetically Encoded FRET Sensor for Cytosolic and Intraorganelle Zn(2+) Imaging.
Article in ACS chemical biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Article
- An Ultraresponsive Green Biosensor for Robust in Vivo Imaging of Synaptic Zinc Dynamics.ACS sensors · 2026Article
- Zinc in eye health, retinal biology and disease.Progress in retinal and eye research · 2025Review
- hpGRISZ: a high-performance fluorescent biosensor for in vivo imaging of synaptic zinc dynamics.bioRxiv : the preprint server for biology · 2025Article
- The human zinc-binding cysteine proteome.Cell · 2025Article
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Luminescence Probes in Bio-Applications: From Principle to Practice.Biosensors · 2024Review
- Zinc-chelating BET bromodomain inhibitors equally target islet endocrine cell types.American journal of physiology. Regulatory, integrative and comparative physiology · 2024Article
- Fluorescent Protein-Based Sensors for Detecting Essential Metal Ions across the Tree of Life.ACS sensors · 2024Review
- Cellular zinc metabolism and zinc signaling: from biological functions to diseases and therapeutic targets.Signal transduction and targeted therapy · 2024Review
- Metalation and activation of ZnBiophysics reviews · 2023Review
- Ratiometric Detection of ZnChemistry (Basel, Switzerland) · 2023Article
- Effects of Marginal Zn Excess and Thiamine Deficiency on Microglial N9 Cell Metabolism and Their Interactions with Septal SN56 Cholinergic Cells.International journal of molecular sciences · 2023Article
- Development and Characterization of a Red Fluorescent Protein-Based Sensor RZnP1 for the Detection of Cytosolic ZnACS sensors · 2022Article
- Mutant Flavin-Based Fluorescent Protein Sensors for Detecting Intracellular Zinc and Copper inACS sensors · 2022Article
- Neuronal signalling of zinc: from detection and modulation to function.Open biology · 2022Review
- Ratiometric Bioluminescent Zinc Sensor Proteins to Quantify Serum and Intracellular Free ZnACS chemical biology · 2022Article
- Transition Metals Induce Quenching of Monomeric Near-Infrared Fluorescent Proteins.Biochemistry · 2022Article
- Organelle-Level Labile ZnACS sensors · 2022Article
- Fluorescent Indicators For Biological Imaging of Monatomic Ions.Frontiers in cell and developmental biology · 2022Review
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7 authors.
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Abstract
Zn(2+) plays essential and diverse roles in numerous cellular processes. To get a better understanding of intracellular Zn(2+) homeostasis and the putative signaling role of Zn(2+), various fluorescent sensors have been developed that allow monitoring of Zn(2+) concentrations in single living cells in real time. Thus far, two families of genetically encoded FRET-based Zn(2+) sensors have been most widely applied, the eCALWY sensors developed by our group and the ZapCY sensors developed by Palmer and co-workers. Both have been successfully used to measure cytosolic free Zn(2+), but distinctly different concentrations have been reported when using these sensors to measure Zn(2+) concentrations in the ER and mitochondria. Here, we report the development of a versatile alternative FRET sensor containing a de novo Cys2His2 binding pocket that was created on the surface of the donor and acceptor fluorescent domains. This eZinCh-2 sensor binds Zn(2+) with a high affinity that is similar to that of eCALWY-4 (Kd = 1 nM at pH 7.1), while displaying a substantially larger change in emission ratio. eZinCh-2 not only provides an attractive alternative for measuring Zn(2+) in the cytosol but was also successfully used for measuring Zn(2+) in the ER, mitochondria, and secretory vesicles. Moreover, organelle-targeted eZinCh-2 can also be used in combination with the previously reported redCALWY sensors to allow multicolor imaging of intracellular Zn(2+) simultaneously in the cytosol and the ER or mitochondria.
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