ArticleMethodsX2014
Efficient purification and concentration of viruses from a large body of high turbidity seawater.
Article in MethodsX, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 42 citations in OpenAlex.
- High-throughput viral enumeration of aquatic ecosystems via flow cytometry.Applied and environmental microbiology · 2026Article
- Metatranscriptomic discovery of a novel viral class from the Yangshan deep-water port virosphere.Archives of virology · 2026Article
- From microscale to microbial insights: validating high-throughput microvolume extraction (HiMEx) methods for marine microbial ecology.ISME communications · 2025Article
- Large-scale single-virus genomics uncovers hidden diversity of river water viruses and diversified gene profiles.The ISME journal · 2024Article
- Metavirome-assembled genome sequence of a new aquatic RNA virus expands the genus Locarnavirus.Archives of virology · 2023Article
- Review
- Isolation and Identification of a Large Green Alga Virus (Journal of virology · 2022Article
- An Interplay between Viruses and Bacteria Associated with the White Sea Sponges Revealed by Metagenomics.Life (Basel, Switzerland) · 2021Article
- Viral Characteristics of the Warm Atlantic and Cold Arctic Water Masses in the Nordic Seas.Applied and environmental microbiology · 2021Article
- Review
- Analysis of Different Size Fractions Provides a More Complete Perspective of Viral Diversity in a Freshwater Embayment.Applied and environmental microbiology · 2021Article
- Viral ecogenomics across the Porifera.Microbiome · 2020Article
- Doubling of the known set of RNA viruses by metagenomic analysis of an aquatic virome.Nature microbiology · 2020Article
- Diverse and unique viruses discovered in the surface water of the East China Sea.BMC genomics · 2020Article
- Article
- Metagenomic Analysis of the Diversity of DNA Viruses in the Surface and Deep Sea of the South China Sea.Frontiers in microbiology · 2019Article
- A new chemical complex can rapidly concentrate lentivirus and significantly enhance gene transduction.Cytotechnology · 2018Article
- Viral Diversity and Its Relationship With Environmental Factors at the Surface and Deep Sea of Prydz Bay, Antarctica.Frontiers in microbiology · 2018Article
- Characterization and prevalence of a novel white spot syndrome viral genotype in naturally infected wild crayfish,Virusdisease · 2017Article
- A rapid Orthopoxvirus purification protocol suitable for high-containment laboratories.Journal of virological methods · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Marine viruses are the most abundant entities in the ocean and play crucial roles in the marine ecological system. However, understanding of viral diversity on large scale depends on efficient and reliable viral purification and concentration techniques. Here, we report on developing an efficient method to purify and concentrate viruses from large body of high turbidity seawater. The developed method characterizes with high viral recovery efficiency, high concentration factor, high viral particle densities and high-throughput, and is reliable for viral concentration from high turbidity seawater. Recovered viral particles were used directly for subsequent analysis by epifluorescence microscopy, transmission electron microscopy and metagenomic sequencing. Three points are essential for this method:•The sampled seawater (>150 L) was initially divided into two parts, water fraction and settled matter fraction, after natural sedimentation.•Both viruses in the water fraction concentrated by tangential flow filtration (TFF) and viruses isolated from the settled matter fraction were considered as the whole viral community in high turbidity seawater.•The viral concentrates were re-concentrated by using centrifugal filter device in order to obtain high density of viral particles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.