ArticleMethodsX2014
Standardized chemical synthesis of Pseudomonas aeruginosa pyocyanin.
Article in MethodsX, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Discovery of a phenazine-thiol conjugase from sparse data using genome-informed machine learning.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Optimization of Pyocyanin Production byAntibiotics (Basel, Switzerland) · 2026Article
- Discovery of a phenazine-thiol conjugase from sparse data using genome-informed machine learning.bioRxiv : the preprint server for biology · 2026Article
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- NADH dehydrogenases are the predominant phenazine reductases in the electron transport chain of Pseudomonas aeruginosa.Molecular microbiology · 2023Article
- The two faces of pyocyanin - why and how to steer its production?World journal of microbiology & biotechnology · 2023Review
- Thermal Pyocyanin Sensor Based on Molecularly Imprinted Polymers for the Indirect Detection ofACS sensors · 2023Article
- Phenazines and toxoflavin act as interspecies modulators of resilience to diverse antibiotics.Molecular microbiology · 2022Article
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- Biosynthesis and metabolic engineering of 1-hydroxyphenazine in Pseudomonas chlororaphis H18.Microbial cell factories · 2021Article
- Computationally designed pyocyanin demethylase acts synergistically with tobramycin to kill recalcitrantProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Bacterial defenses against a natural antibiotic promote collateral resilience to clinical antibiotics.PLoS biology · 2021Article
- Phenazines as potential biomarkers of Pseudomonas aeruginosa infections: synthesis regulation, pathogenesis and analytical methods for their detection.Analytical and bioanalytical chemistry · 2020Review
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- The transcription factors ActR and SoxR differentially affect the phenazine tolerance of Agrobacterium tumefaciens.Molecular microbiology · 2019Article
- Both toxic and beneficial effects of pyocyanin contribute to the lifecycle of Pseudomonas aeruginosa.Molecular microbiology · 2018Article
- Transient Proteotoxicity of Bacterial Virulence Factor Pyocyanin in Renal Tubular Epithelial Cells Induces ER-Related Vacuolation and Can Be Efficiently Modulated by Iron Chelators.Toxicological sciences : an official journal of the Society of Toxicology · 2016Article
- Genetic engineering of Pseudomonas chlororaphis GP72 for the enhanced production of 2-Hydroxyphenazine.Microbial cell factories · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Preparation of the toxin pyocyanin from the bacterium Pseudomonas aeruginosa is an exacting procedure. Pyocyanin is expensive to commercially purchase. The sellers do not give out the extraction procedure. Classically, pyocyanin preparation involves complicated multi-step P. aeruginosa culturing and solvent transfer extractions. The chemical synthesis first used (1979) has not been adequately described. We devised an easily reproducible protocol which consistently decreases the time taken for synthesis, extraction and purification of pyocyanin, and increases the pure pyocyanin proportion produced. Our procedure:•Involves more purification steps (chloroform/methanol/acidification/alkalinization).•Starts with a different pH (7.4 instead of 7), and lesser concentration of phenazine methosulfate; and retrenches a rotary evaporation step.•Removes 2 lyophilization steps, and entails different solvent proportions for thin layer chromatography. As we have extracted pyocyanin both from P. aeruginosa cultures, and via chemical synthesis; we know the procedural and product-quality differences. We endorse the relative ease, safety, and convenience of using the chemical synthesis described here. Crucially, our "naturally endotoxin-free" pyocyanin can be extracted easily without using infectious bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.