ArticlePhilosophical transactions of the Royal Society of London. Series B, Biological sciences2015
Within-host competition between Borrelia afzelii ospC strains in wild hosts as revealed by massively parallel amplicon sequencing.
Article in Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
- Tick-to-host transmission differs betweenMicrobiology spectrum · 2023Article
- Variation among strains of Borrelia burgdorferi in host tissue abundance and lifetime transmission determine the population strain structure in nature.PLoS pathogens · 2023Article
- MHC class II genotype-by-pathogen genotype interaction for infection prevalence in a natural rodent-Borrelia system.Evolution; international journal of organic evolution · 2022Article
- Divergence in Coding Sequence and Expression of Different Functional Categories of Immune Genes between Two Wild Rodent Species.Genome biology and evolution · 2021Article
- Perpetuation of Borreliae.Current issues in molecular biology · 2021Article
- Getting under the birds' skin: tissue tropism of Borrelia burgdorferi s.l. in naturally and experimentally infected avian hosts.Microbial ecology · 2020Article
- Deconvoluting the diversity of within-host pathogen strains in a multi-locus sequence typing framework.BMC bioinformatics · 2019Article
- Susceptibility to infection with Borrelia afzelii and TLR2 polymorphism in a wild reservoir host.Scientific reports · 2019Article
- Competition Between Strains ofFrontiers in cellular and infection microbiology · 2019Article
- Ixodes scapularis does not harbor a stable midgut microbiome.The ISME journal · 2018Article
- Competition between strains ofProceedings. Biological sciences · 2018Article
- Emergence of tick-borne diseases at northern latitudes in Europe: a comparative approach.Scientific reports · 2017Article
- Ticks infected via co-feeding transmission can transmit Lyme borreliosis to vertebrate hosts.Scientific reports · 2017Article
- Fitness estimates from experimental infections predict the long-term strain structure of a vector-borne pathogen in the field.Scientific reports · 2017Article
- Multistrain Infections with Lyme Borreliosis Pathogens in the Tick Vector.Applied and environmental microbiology · 2017Article
- Inefficient co-feeding transmission of Borrelia afzelii in two common European songbirds.Scientific reports · 2017Article
- Comparison of the lifetime host-to-tick transmission between two strains of the Lyme disease pathogen Borrelia afzelii.Parasites & vectors · 2016Article
- Vectors as Epidemiological Sentinels: Patterns of Within-Tick Borrelia burgdorferi Diversity.PLoS pathogens · 2016Article
- Article
- Cross-Immunity and Community Structure of a Multiple-Strain Pathogen in the Tick Vector.Applied and environmental microbiology · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Infections frequently consist of more than one strain of a given pathogen. Experiments have shown that co-infecting strains often compete, so that the infection intensity of each strain in mixed infections is lower than in single strain infections. Such within-host competition can have important epidemiological and evolutionary consequences. However, the extent of competition has rarely been investigated in wild, naturally infected hosts, where there is noise in the form of varying inoculation doses, asynchronous infections and host heterogeneity, which can potentially alleviate or eliminate competition. Here, we investigated the extent of competition between Borrelia afzelii strains (as determined by ospC genotype) in three host species sampled in the wild. For this purpose, we developed a protocol for 454 amplicon sequencing of ospC, which allows both detection and quantification of each individual strain in an infection. Each host individual was infected with one to six ospC strains. The infection intensity of each strain was lower in mixed infections than in single ones, showing that there was competition. Rank-abundance plots revealed that there was typically one dominant strain, but that the evenness of the relative infection intensity of the different strains in an infection increased with the multiplicity of infection. We conclude that within-host competition can play an important role under natural conditions despite many potential sources of noise, and that quantification by next-generation amplicon sequencing offers new possibilities to dissect within-host interactions in naturally infected hosts.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.