Evidence map›Paper›PMID 26150541›Full record

ArticleInfection and immunity2015

Heparan Sulfate Modulates Neutrophil and Endothelial Function in Antibacterial Innate Immunity.

Ding Xu, Joshua Olson, Jason N Cole, Xander M van Wijk, Volker Brinkmann, Arturo Zychlinsky, Victor Nizet, Jeffrey D Esko, Yung-Chi Chang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

  1. Heparan Sulfate Proteoglycans in Immunity.Proteoglycan research · 2026
    Article
  2. Article
  3. Article
  4. Review
  5. Article
  6. Review
  7. Neutrophil Extracellular Traps inFrontiers in immunology · 2022
    Review
  8. Article
  9. Article
  10. Article
  11. Review
  12. Synthesis of 3-Biochemistry · 2020
    Article
  13. Article
  14. Loss of endothelial sulfatase-1 after experimental sepsis attenuates subsequent pulmonary inflammatory responses.American journal of physiology. Lung cellular and molecular physiology · 2019
    Article
  15. Review
  16. Review
  17. The heparan sulfate proteoglycan grip on hyperlipidemia and atherosclerosis.Matrix biology : journal of the International Society for Matrix Biology · 2018
    Review
  18. Article
  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ding XuGlycobiology Research and Training Center, University of California, San Diego, La Jolla, California, USA Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California, USA Department of Oral Biology, University at Buffalo, School of Dental Medicine, The State University of New York, Buffalo, New York, USA.
Joshua OlsonDepartment of Pediatrics, University of California, San Diego, La Jolla, California, USA.
Jason N ColeGlycobiology Research and Training Center, University of California, San Diego, La Jolla, California, USA Department of Pediatrics, University of California, San Diego, La Jolla, California, USA School of Chemistry and Molecular Biosciences and Australian Infectious Diseases Research Centre, The University of Queensland, St. Lucia, Queensland, Australia.
Xander M van WijkGlycobiology Research and Training Center, University of California, San Diego, La Jolla, California, USA Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California, USA.
Volker BrinkmannDepartment of Cellular Microbiology, Max Planck Institute for Infection Biology, Berlin, Germany.
Arturo ZychlinskyDepartment of Cellular Microbiology, Max Planck Institute for Infection Biology, Berlin, Germany.
Victor NizetGlycobiology Research and Training Center, University of California, San Diego, La Jolla, California, USA Department of Pediatrics, University of California, San Diego, La Jolla, California, USA Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California, San Diego, La Jolla, California, USA.
Jeffrey D EskoGlycobiology Research and Training Center, University of California, San Diego, La Jolla, California, USA Department of Cellular and Molecular Medicine, University of California, San Diego, La Jolla, California, USA jesko@ucsd.edu yungchiychang@ntu.edu.tw.
Yung-Chi ChangGlycobiology Research and Training Center, University of California, San Diego, La Jolla, California, USA Department of Pediatrics, University of California, San Diego, La Jolla, California, USA Graduate Institute of Microbiology, College of Medicine, National Taiwan University, Taipei, Taiwan jesko@ucsd.edu yungchiychang@ntu.edu.tw.

Funding

Siglec Modulation of Inflammatory ResponsesP01HL107150 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI LE, DZUNG T · 2011 to 2017
$18.0M
U of Calif, San Diego Neuroscience Microscopy ImagingP30NS047101 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GLEESON, JOSEPH G, ZHENG, BINHAI · 2003 to 2022
$9.0M
GAS Switch from Colonizing Bacterium to Invasive PathogenR01AI077780 · NIAID · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NIZET, VICTOR · 2008 to 2019
$3.8M
Glycoprotein Remodeling in the Vasculopathy and Coagulopathy of SepsisR01HL125352 · NHLBI · UNIVERSITY OF CALIFORNIA SANTA BARBARA · PI MARTH, JAMEY, NIZET, VICTOR · 2014 to 2018
$3.6M
NHLBI NIH HHS HL107150NHLBI NIH HHS P01 HL107150NHLBI NIH HHS R01 HL125352NIAID NIH HHS R01 AI077780NINDS NIH HHS P30 NS047101
6 · The paper itself

Abstract

Recently, we showed that endothelial heparan sulfate facilitates entry of a bacterial pathogen into the central nervous system. Here, we show that normal bactericidal activity of neutrophils is influenced by the sulfation pattern of heparan sulfate. Inactivation of heparan sulfate uronyl 2-O-sulfotransferase (Hs2st) in neutrophils substantially reduced their bactericidal activity, and Hs2st deficiency rendered mice more susceptible to systemic infection with the pathogenic bacterium group B Streptococcus. Specifically, altered sulfation of heparan sulfate in mutant neutrophils affected formation of neutrophil extracellular traps while not influencing phagocytosis, production of reactive oxygen species, or secretion of granular proteases. Heparan sulfate proteoglycan(s) is present in neutrophil extracellular traps, modulates histone affinity, and modulates their microbial activity. Hs2st-deficient brain endothelial cells show enhanced binding to group B Streptococcus and are more susceptible to apoptosis, likely contributing to the observed increase in dissemination of group B Streptococcus into the brain of Hs2st-deficient mice following intravenous challenge. Taken together, our data provide strong evidence that heparan sulfate from both neutrophils and the endothelium plays important roles in modulating innate immunity.

Indexed as

AnimalsBlotting, WesternCells, CulturedDisease Models, AnimalEndothelial CellsExtracellular TrapsHeparan Sulfate ProteoglycansImmunity, InnateMiceMicroscopy, Electron, ScanningNeutrophilsStreptococcal InfectionsStreptococcus agalactiaeSulfotransferasesheparan-sulfate 2-sulfotransferaseHeparan Sulfate ProteoglycansSulfotransferases

Identifiers

PMID26150541
PMCPMC4534644

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.