ArticleApplied and environmental microbiology2015
Detection of Low-Level Cardinium and Wolbachia Infections in Culicoides.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 64 citations in OpenAlex.
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- Characterization of the Bacteriome ofInsects · 2026Article
- Extracellular microbes are required for mosquito development even in the presence of Wolbachia.PLoS neglected tropical diseases · 2025Article
- Characterisation of the virome ofThe Journal of general virology · 2025Article
- Wolbachia infection in natural mosquito populations from Argentina.Parasitology research · 2024Article
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- Detection and quantification of naturalFrontiers in cellular and infection microbiology · 2024Article
- Biting Midges (Diptera: Ceratopogonidae) as Vectors of Viruses.Microorganisms · 2023Review
- Beyond canonical models: why a broader understanding of Diptera-microbiota interactions is essential for vector-borne disease control.Evolutionary ecology · 2023Article
- Wolbachia wAlbB inhibits bluetongue and epizootic hemorrhagic fever viruses in Culicoides midge cells.Medical and veterinary entomology · 2022Article
- Species Identity, Life History, and Geographic Distance Influence Gut Bacterial Communities in Lab-Reared and European Field-Collected Culicoides Biting midges.Microbial ecology · 2022Article
- Discovery of Early-BranchingFrontiers in microbiology · 2022Article
- Molecular phylogeny of heritable symbionts and microbiota diversity analysis in phlebotominae sand flies and Culex nigripalpus from Colombia.PLoS neglected tropical diseases · 2021Article
- Wolbachia: endosymbiont of onchocercid nematodes and their vectors.Parasites & vectors · 2021Review
- Next-generation tools to control biting midge populations and reduce pathogen transmission.Parasites & vectors · 2021Review
- Tissue Tropisms and Transstadial Transmission of aApplied and environmental microbiology · 2020Article
- Sex-SpecificInsects · 2020Article
- Phenotypic Response ofMicroorganisms · 2020Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial endosymbionts have been identified as potentially useful biological control agents for a range of invertebrate vectors of disease. Previous studies of Culicoides (Diptera: Ceratopogonidae) species using conventional PCR assays have provided evidence of Wolbachia (1/33) and Cardinium (8/33) infections. Here, we screened 20 species of Culicoides for Wolbachia and Cardinium, utilizing a combination of conventional PCR and more sensitive quantitative PCR (qPCR) assays. Low levels of Cardinium DNA were detected in females of all but one of the Culicoides species screened, and low levels of Wolbachia were detected in females of 9 of the 20 Culicoides species. Sequence analysis based on partial 16S rRNA gene and gyrB sequences identified "Candidatus Cardinium hertigii" from group C, which has previously been identified in Culicoides from Japan, Israel, and the United Kingdom. Wolbachia strains detected in this study showed 98 to 99% sequence identity to Wolbachia previously detected from Culicoides based on the 16S rRNA gene, whereas a strain with a novel wsp sequence was identified in Culicoides narrabeenensis. Cardinium isolates grouped to geographical regions independent of the host Culicoides species, suggesting possible geographical barriers to Cardinium movement. Screening also identified Asaia bacteria in Culicoides. These findings point to a diversity of low-level endosymbiont infections in Culicoides, providing candidates for further characterization and highlighting the widespread occurrence of these endosymbionts in this insect group.
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Registered trials
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