Evidence map›Paper›PMID 26150419›Full record

ArticleNucleic acids research2015

Transcriptomic profiling of gene expression and RNA processing during Leishmania major differentiation.

Laura A L Dillon, Kwame Okrah, V Keith Hughitt, Rahul Suresh, Yuan Li, Maria Cecilia Fernandes, A Trey Belew, Hector Corrada Bravo, David M Mosser, Najib M El-Sayed

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.

0numbers the graph read from it
0cells of the map it votes in
54citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

54 citing papers in PubMed.

  1. Article
  2. RNA synthesis inMicrobiology spectrum · 2025
    Article
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  6. Single-cell atlas ofProceedings of the National Academy of Sciences of the United States of America · 2024
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  7. Article
  8. Article
  9. Review
  10. Article
  11. Promastigote-to-Amastigote Conversion inPathogens (Basel, Switzerland) · 2022
    Review
  12. New Insights on Heme Uptake inInternational journal of molecular sciences · 2022
    Article
  13. Article
  14. Immune Responses in Leishmaniasis: An Overview.Tropical medicine and infectious disease · 2022
    Review
  15. Article
  16. Finding Correlations Between mRNA and Protein Levels inFrontiers in cellular and infection microbiology · 2022
    Review
  17. Catalase impairsVirulence · 2021
    Article
  18. Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Laura A L DillonDepartment of Cell Biology and Molecular Genetics, 3128 Bioscience Research Building, University of Maryland, College Park, MD 20742, USA Center for Bioinformatics and Computational Biology, University of Maryland, College Park, MD 20742, USA.
Kwame OkrahDepartment of Mathematics, University of Maryland, College Park, MD 20742, USA.
V Keith HughittDepartment of Cell Biology and Molecular Genetics, 3128 Bioscience Research Building, University of Maryland, College Park, MD 20742, USA Center for Bioinformatics and Computational Biology, University of Maryland, College Park, MD 20742, USA.
Rahul SureshDepartment of Cell Biology and Molecular Genetics, 3128 Bioscience Research Building, University of Maryland, College Park, MD 20742, USA.
Yuan LiDepartment of Cell Biology and Molecular Genetics, 3128 Bioscience Research Building, University of Maryland, College Park, MD 20742, USA Center for Bioinformatics and Computational Biology, University of Maryland, College Park, MD 20742, USA.
Maria Cecilia FernandesDepartment of Cell Biology and Molecular Genetics, 3128 Bioscience Research Building, University of Maryland, College Park, MD 20742, USA Center for Bioinformatics and Computational Biology, University of Maryland, College Park, MD 20742, USA.
A Trey BelewDepartment of Cell Biology and Molecular Genetics, 3128 Bioscience Research Building, University of Maryland, College Park, MD 20742, USA Center for Bioinformatics and Computational Biology, University of Maryland, College Park, MD 20742, USA.
Hector Corrada BravoCenter for Bioinformatics and Computational Biology, University of Maryland, College Park, MD 20742, USA Department of Computer Science, University of Maryland, College Park, MD 20742, USA.
David M MosserDepartment of Cell Biology and Molecular Genetics, 3128 Bioscience Research Building, University of Maryland, College Park, MD 20742, USA.
Najib M El-SayedDepartment of Cell Biology and Molecular Genetics, 3128 Bioscience Research Building, University of Maryland, College Park, MD 20742, USA Center for Bioinformatics and Computational Biology, University of Maryland, College Park, MD 20742, USA elsayed@umd.edu.

Funding

Profiling the Leishmania-macrophage Host-pathogen InfectomeR01AI094773 · NIAID · UNIV OF MARYLAND, COLLEGE PARK · PI EL-SAYED, NAJIB M, MOSSER, DAVID M · 2011 to 2014
$2.5M
Regulatory macrophages and the host inflammatory responseR01GM102589 · NIGMS · UNIV OF MARYLAND, COLLEGE PARK · PI MOSSER, DAVID M · 2013 to 2016
$1.2M
NIAID NIH HHS AI094773NIAID NIH HHS R01 AI094773NIGMS NIH HHS R01 GM102589
6 · The paper itself

Abstract

Protozoan parasites of the genus Leishmania are the etiological agents of leishmaniasis, a group of diseases with a worldwide incidence of 0.9-1.6 million cases per year. We used RNA-seq to conduct a high-resolution transcriptomic analysis of the global changes in gene expression and RNA processing events that occur as L. major transforms from non-infective procyclic promastigotes to infective metacyclic promastigotes. Careful statistical analysis across multiple biological replicates and the removal of batch effects provided a high quality framework for comprehensively analyzing differential gene expression and transcriptome remodeling in this pathogen as it acquires its infectivity. We also identified precise 5' and 3' UTR boundaries for a majority of Leishmania genes and detected widespread alternative trans-splicing and polyadenylation. An investigation of possible correlations between stage-specific preferential trans-splicing or polyadenylation sites and differentially expressed genes revealed a lack of systematic association, establishing that differences in expression levels cannot be attributed to stage-regulated alternative RNA processing. Our findings build on and improve existing expression datasets and provide a substantially more detailed view of L. major biology that will inform the field and potentially provide a stronger basis for drug discovery and vaccine development efforts.

Indexed as

Gene Expression Regulation, DevelopmentalRNA Processing, Post-TranscriptionalGene Expression ProfilingGene OntologyGenes, ProtozoanLeishmania majorPolyadenylationSequence Analysis, RNATrans-Splicing

Identifiers

PMID26150419
PMCPMC4538839

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.