ArticleUltrasonics2015
Acoustic tweezers for studying intracellular calcium signaling in SKBR-3 human breast cancer cells.
Article in Ultrasonics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
- Recent Advancements in High-Frequency Ultrasound Applications from Imaging to Microbeam Stimulation.Sensors (Basel, Switzerland) · 2024Review
- Viscoelasticity in 3D Cell Culture and Regenerative Medicine: Measurement Techniques and Biological Relevance.ACS materials Au · 2024Review
- Ultrasonic High-Resolution Imaging and Acoustic Tweezers Using Ultrahigh Frequency Transducer: Integrative Single-Cell Analysis.Sensors (Basel, Switzerland) · 2023Article
- Automated cell-type classification combining dilated convolutional neural networks with label-free acoustic sensing.Scientific reports · 2022Article
- Manipulation and Mechanical Deformation of Leukemia Cells by High-Frequency Ultrasound Single Beam.IEEE transactions on ultrasonics, ferroelectrics, and frequency control · 2022Article
- Automated estimation of cancer cell deformability with machine learning and acoustic trapping.Scientific reports · 2022Article
- Recent Advancements in Ultrasound Transducer: From Material Strategies to Biomedical Applications.BME frontiers · 2022Review
- Measurements of acoustic radiation force of ultrahigh frequency ultrasonic transducers using model-based approach.Applied physics letters · 2021Article
- Article
- Integrin Antibody Decreases Deformability of Patient-Derived Pre-B Acute Lymphocytic Leukemia Cells as Measured by High-Frequency Acoustic Tweezers.Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine · 2020Article
- Investigation of cell mechanics using single-beam acoustic tweezers as a versatile tool for the diagnosis and treatment of highly invasive breast cancer cell lines: an in vitro study.Microsystems & nanoengineering · 2020Article
- Characterizing Deformability of Drug Resistant Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells Using Acoustic Tweezers.Scientific reports · 2018Article
- Label-free analysis of the characteristics of a single cell trapped by acoustic tweezers.Scientific reports · 2017Article
- Quantification of Inter-Erythrocyte Forces with Ultra-High Frequency (410 MHz) Single Beam Acoustic Tweezer.Annals of biomedical engineering · 2017Article
- Direct and sustained intracellular delivery of exogenous molecules using acoustic-transfection with high frequency ultrasound.Scientific reports · 2016Article
- Impedance matching network for high frequency ultrasonic transducer for cellular applications.Ultrasonics · 2016Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
Extracellular matrix proteins such as fibronectin (FNT) play crucial roles in cell proliferation, adhesion, and migration. For better understanding of these associated cellular activities, various microscopic manipulation tools have been used to study their intracellular signaling pathways. Recently, it has appeared that acoustic tweezers may possess similar capabilities in the study. Therefore, we here demonstrate that our newly developed acoustic tweezers with a high-frequency lithium niobate ultrasonic transducer have potentials to study intracellular calcium signaling by FNT-binding to human breast cancer cells (SKBR-3). It is found that intracellular calcium elevations in SKBR-3 cells, initially occurring on the microbead-contacted spot and then eventually spreading over the entire cell, are elicited by attaching an acoustically trapped FNT-coated microbead. Interestingly, they are suppressed by either extracellular calcium elimination or phospholipase C (PLC) inhibition. Hence, this suggests that our acoustic tweezers may serve as an alternative tool in the study of intracellular signaling by FNT-binding activities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.