Evidence map›Paper›PMID 26150232›Full record

ArticleMolecular systems biology2015

Pom1 gradient buffering through intermolecular auto-phosphorylation.

Micha Hersch, Olivier Hachet, Sascha Dalessi, Pranav Ullal, Payal Bhatia, Sven Bergmann, Sophie G Martin

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular systems biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.1field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 22 citations in OpenAlex.

  1. Review
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  3. Molecular Mechanism of Cytokinesis.Annual review of biochemistry · 2019
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  4. Article
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  7. Nine unanswered questions about cytokinesis.The Journal of cell biology · 2017
    Review
  8. Controlling cell size through sizer mechanisms.Current opinion in systems biology · 2017
    Review
  9. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Micha HerschDepartment of Medical Genetics, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics, Lausanne, Switzerland.
Olivier HachetDepartment of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland.
Sascha DalessiDepartment of Medical Genetics, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics, Lausanne, Switzerland.
Pranav UllalDepartment of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland.
Payal BhatiaDepartment of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland.
Sven BergmannDepartment of Medical Genetics, University of Lausanne, Lausanne, Switzerland Swiss Institute of Bioinformatics, Lausanne, Switzerland sophie.martin@unil.ch sven.bergmann@unil.ch.
Sophie G MartinDepartment of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland sophie.martin@unil.ch sven.bergmann@unil.ch.
University of Lausanne · CHSIB Swiss Institute of Bioinformatics · CH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Concentration gradients provide spatial information for tissue patterning and cell organization, and their robustness under natural fluctuations is an evolutionary advantage. In rod-shaped Schizosaccharomyces pombe cells, the DYRK-family kinase Pom1 gradients control cell division timing and placement. Upon dephosphorylation by a Tea4-phosphatase complex, Pom1 associates with the plasma membrane at cell poles, where it diffuses and detaches upon auto-phosphorylation. Here, we demonstrate that Pom1 auto-phosphorylates intermolecularly, both in vitro and in vivo, which confers robustness to the gradient. Quantitative imaging reveals this robustness through two system's properties: The Pom1 gradient amplitude is inversely correlated with its decay length and is buffered against fluctuations in Tea4 levels. A theoretical model of Pom1 gradient formation through intermolecular auto-phosphorylation predicts both properties qualitatively and quantitatively. This provides a telling example where gradient robustness through super-linear decay, a principle hypothesized a decade ago, is achieved through autocatalysis. Concentration-dependent autocatalysis may be a widely used simple feedback to buffer biological activities.

Indexed as

AlgorithmsCell DivisionCell MembraneMicrotubule-Associated ProteinsPhosphorylationProtein KinasesSchizosaccharomycesSchizosaccharomyces pombe ProteinsMicrotubule-Associated ProteinsPom1 protein, S pombeProtein KinasesSchizosaccharomyces pombe ProteinsTea4 protein, S pombeauto‐catalysiscell cycle controlfission yeast Schizosaccharomyces pombegradient formationrobustness

Identifiers

PMID26150232
PMCPMC4547846
OpenAlexW2157732527

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.