ArticleAntimicrobial agents and chemotherapy2015
Pyoverdine and proteases affect the response of Pseudomonas aeruginosa to gallium in human serum.
Article in Antimicrobial agents and chemotherapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- GaSal-2: A Water-Soluble Antipseudomonal Agent Targeting the Extracellular Hemophore HasAp.ACS infectious diseases · 2025Article
- Gallium: a decisive "Trojan Horse" against microorganisms.Antonie van Leeuwenhoek · 2024Review
- The diadenosine tetraphosphate hydrolase ApaH contributes to Pseudomonas aeruginosa pathogenicity.PLoS pathogens · 2024Article
- Ga(III) pyridinecarboxylate complexes: potential analogues of the second generation of therapeutic Ga(III) complexes?Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2023Article
- A DFT study of the gallium ion-binding capacity of mature Pseudomonas aeruginosa biofilm extracellular polysaccharide.PloS one · 2023Article
- Novel Insights on Pyoverdine: From Biosynthesis to Biotechnological Application.International journal of molecular sciences · 2022Review
- Variable Susceptibility to Gallium Compounds of Major Cystic Fibrosis Pathogens.ACS infectious diseases · 2022Article
- Treatment ofFrontiers in microbiology · 2022Review
- Article
- Biofilm-specific uptake of a 4-pyridone-based iron chelator by Pseudomonas aeruginosa.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2021Article
- The Antibacterial and Anti-biofilm Activity of Metal Complexes Incorporating 3,6,9-Trioxaundecanedioate and 1,10-Phenanthroline Ligands in Clinical Isolates ofAntibiotics (Basel, Switzerland) · 2020Article
- N-acetyl Cysteine Coated Gallium Particles Demonstrate High Potency againstPathogens (Basel, Switzerland) · 2019Article
- Activity and Impact on Resistance Development of Two Antivirulence Fluoropyrimidine Drugs inFrontiers in cellular and infection microbiology · 2019Article
- Drug Repurposing for the Treatment of Bacterial and Fungal Infections.Frontiers in microbiology · 2019Review
- Low spatial structure and selection against secreted virulence factors attenuates pathogenicity in Pseudomonas aeruginosa.The ISME journal · 2018Article
- Novel Pyoverdine Inhibitors MitigateFrontiers in microbiology · 2018Article
- Probing the evolutionary robustness of two repurposed drugs targeting iron uptake inEvolution, medicine, and public health · 2018Article
- Antimicrobial Activity of Gallium Compounds on ESKAPE Pathogens.Frontiers in cellular and infection microbiology · 2018Article
- Ferric Uptake Regulator Fur Is Conditionally Essential in Pseudomonas aeruginosa.Journal of bacteriology · 2017Article
- A short D-enantiomeric antimicrobial peptide with potent immunomodulatory and antibiofilm activity against multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gallium is an iron mimetic which has recently been repurposed as an antibacterial agent due to its capability to disrupt bacterial iron metabolism. In this study, the antibacterial activity of gallium nitrate [Ga(NO3)3] was investigated in complement-free human serum (HS) on 55 Pseudomonas aeruginosa clinical isolates from cystic fibrosis and non-cystic fibrosis patients. The susceptibility of P. aeruginosa to Ga(NO3)3 in HS was dependent on the bacterial ability to acquire iron from serum binding proteins (i.e., transferrin). The extent of serum protein degradation correlated well with P. aeruginosa growth in HS, while pyoverdine production did not. However, pyoverdine-deficient P. aeruginosa strains were unable to grow in HS and overcome iron restriction, albeit capable of releasing proteases. Predigestion of HS with proteinase K promoted the growth of all strains, irrespective of their ability to produce proteases and/or pyoverdine. The MICs of Ga(NO3)3 were higher in HS than in an iron-poor Casamino Acids medium, where proteolysis does not affect iron availability. Coherently, strains displaying high proteolytic activity were less susceptible to Ga(NO3)3 in HS. Our data support a model in which both pyoverdine and proteases affect the response of P. aeruginosa to Ga(NO3)3 in HS. The relatively high Ga(NO3)3 concentration required to inhibit the growth of highly proteolytic P. aeruginosa isolates in HS poses a limitation to the potential of Ga(NO3)3 in the treatment of P. aeruginosa bloodstream infections.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.