ArticleOecologia2015
Experimental fertilization increases amino acid content in floral nectar, fruit set and degree of selfing in the orchid Gymnadenia conopsea.
Article in Oecologia, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Floral Color and Family Drive Contrasting Plant-Pollinator Responses to Nutrient Enrichment.Ecology and evolution · 2025Article
- Effects of Climate Change Scenarios on Growth, Flowering Characteristics, and Honey Production Potential ofPlants (Basel, Switzerland) · 2025Article
- Do pollinators play a role in shaping the essential amino acids found in nectar?The New phytologist · 2025Article
- Trade-off between pollinator-wildflower diversity & grassland yields.npj biodiversity · 2025Article
- Origin and Function of Amino Acids in Nectar and Nectaries ofPlants (Basel, Switzerland) · 2023Article
- Nitrogen addition affects floral and vegetative traits, reproduction, and pollinator performance in Capsicum annuum L.Annals of botany · 2023Article
- Nitrogen Addition Affects Interannual Variation in Seed Production in a Tibetan Perennial Herb.Biology · 2023Article
- In Which Way Do the Flower Properties of the Specialist OrchidInternational journal of molecular sciences · 2023Article
- Does Reproductive Success in Natural and Anthropogenic Populations of GeneralistInternational journal of molecular sciences · 2023Article
- Pollen transfer and patterns of reproductive success in pure and mixed populations of nectariferousPeerJ · 2022Article
- Comparative analyses of the metabolite and ion concentrations in nectar, nectaries, and leaves of 36 bromeliads with different photosynthesis and pollinator types.Frontiers in plant science · 2022Article
- Nectar Chemistry or Flower Morphology-What Is More Important for the Reproductive Success of Generalist OrchidInternational journal of molecular sciences · 2021Article
- Floral Nectar Chemistry in Orchids: A Short Review and Meta-Analysis.Plants (Basel, Switzerland) · 2021Article
- Bumble Bee Foraged Pollen Analyses in Spring Time in Southern Estonia Shows Abundant Food Sources.Insects · 2021Article
- Effects of fertilisation on grass and forb gamic reproduction in semi-natural grasslands.Scientific reports · 2021Article
- The role of soils on pollination and seed dispersal.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2021Review
- Nectar biosynthesis is conserved among floral and extrafloral nectaries.Plant physiology · 2021Article
- How Are the Flower Structure and Nectar Composition of the Generalistic OrchidInternational journal of molecular sciences · 2021Article
- Intraspecific Variation in Nectar Chemistry and Its Implications for Insect Visitors: The Case of the Medicinal Plant,Plants (Basel, Switzerland) · 2020Article
- Below-ground competition alters attractiveness of an insect-pollinated plant to pollinators.AoB PLANTS · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Floral traits have evolved to maximize reproductive success by attracting pollinators and facilitating pollination. Highly attractive floral traits may, however, also increase the degree of self-pollination, which could become detrimental for plant fitness through inbreeding depression. Floral nectar is a trait that is known to strongly mediate pollinator attraction and plant reproductive success, but the particular role of the nectar amino acid (AA) composition is poorly understood. Therefore, we experimentally manipulated the nectar AA composition and abundance of the Lepidoptera-pollinated orchid Gymnadenia conopsea through soil fertilization, and we quantified AA content and AA composition through high performance anion exchange chromatography with pulsed amperometric detection. Mixed models were then used to evaluate differences in pollinia removal, fruit set, seed set and degree of selfing between fertilized and control individuals. Selfing rates were estimated using microsatellite markers. We found that fertilized individuals had a significantly higher nectar AA content and an altered AA composition, whereas plant height, number of flowers, nectar volume and sugar concentration remained unchanged. Fertilized individuals also had significantly more pollinia removed and a higher fruit set, whereas control plants that did not receive the fertilization treatment had significantly fewer selfed seeds, and more viable seeds. Although we cannot exclude a role of changes in floral scent following the fertilization treatment, our results strongly suggest a relation among nectar AA composition, fruiting success and selfing rates. Our results also indicate potential consequences of nutrient pollution for plant reproductive success, through the induced changes in nectar AA composition.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.